CTX-0294885 hydrochloride |
| Catalog No.GC35755 |
Le chlorhydrate de CTX-0294885 est un inhibiteur de kinase À large spectre qui peut capturer 235 kinases À partir de cellules MDA-MB-231 et peut capturer tous les membres de la famille AKT.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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CTX-0294885 hydrochloride is a sepharose-supported kinase capture reagent.
CTX-0294885 is a novel bisanilino pyrimidine exhibiting inhibitory activity against a broad range of kinases. CTx-0294885 represents a powerful new reagent for analysis of kinome signaling networks that may facilitate development of targeted therapeutic strategies. Large-scale CTx-0294885-based affinity purification followed by LC-MS/MS leads to the identification of 235 protein kinases from MDA-MB-231 cells, including all members of the AKT family that has not been previously detected by other broad-spectrum kinase inhibitors. Addition of CTx-0294885 to a mixture of three kinase inhibitors commonly used for kinase-enrichment increases the number of kinase identifications to 261. Combining CTx-0294885 with three other broad-specificity inhibitors for kinase enabled the identification of 799 highconfidence phosphosites on 183 kinases, 10% of which are localized to the activation loop, and included previously unreported phosphosites on BMP2K, MELK, HIPK2, and PRKDC[1].
[1]. Zhang L, et al. Characterization of the novel broad-spectrum kinase inhibitor CTx-0294885 as an affinity reagent for mass spectrometry-based kinome profiling. J Proteome Res. 2013 Jul 5;12(7):3104-16.
| Cas No. | SDF | ||
| Canonical SMILES | [H]Cl.O=C(NC)C1=CC=CC=C1NC2=NC(NC3=CC=C(N4CCNCC4)C=C3)=NC=C2Cl | ||
| Formula | C22H25Cl2N7O | M.Wt | 474.39 |
| Solubility | Water: ≥ 370 mg/mL (779.95 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.108 mL | 10.5399 mL | 21.0797 mL |
| 5 mM | 0.4216 mL | 2.108 mL | 4.2159 mL |
| 10 mM | 0.2108 mL | 1.054 mL | 2.108 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
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