(±)-Jasmonic Acid-Isoleucine (Synonyms: (±)-JA-Ile) |
| Catalog No.GC19497 |
JA-Ile est une hormone végétale formée par la conjugaison de l'acide jasmonique et de l'acide aminé isoleucine.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 120330-93-0
Sample solution is provided at 25 µL, 10mM.
(±)-Jasmonic acid-isoleucine (JA-Ile) is a mixture that contains two of the four possible stereoisomers of JA-Ile: (1R,2R)- and (1S,2S)-JA-Ile. JA-Ile is a plant hormone formed by the conjugation of jasmonic acid and the amino acid isoleucine.[1] JA-Ile signals through a co-receptor complex composed of the jasmonate ZIM-domain (JAZ) repressor proteins and an E1 ubiquitin ligase complex containing the F-box Coronatine Insensitive 1 (COI1).[2],[1] Activation of the COI1-JAZ complex directs the degradation of the JAZ protein, resulting in the release of transcription factors bound and inhibited by JAZ.2,1 JA-Ile contributes to several aspects of plant growth and development and levels increase under stress conditions leading to the production of defense compounds and inhibition of growth.[2],[3],[4] The (–)-JA-Ile isomer is more active than the (+)-JA-Ile isomer at inducing gene expression of jasmonate-induced protein of 23 kDa (JIP-23) in barley.[5]
Reference:
[1]. Katsir, L., Chung, H.S., Koo, A.J.K., et al. Jasmonate signaling: A conserved mechanism of hormone sensing Curr. Opin. Plant Biol. 11(4), 428-435 (2008).
[2]. Goossens, J.F., Fernández-Calvo, P., Schwizer, F., et al. Jasmonates: Signal transduction components and their roles in environmental stress responses Plant. Mol. Biol. 91(6), 673-689 (2016).
[3]. Kang, J.-H., Wang, L., Giri, A., et al. Silencing threonine deaminase and JAR4 in Nicotiana attenuata impairs jasmonic acid–isoleucine–mediated defenses against Manduca sexta Plant Cell 18(11), 3303-3320 (2006).
[4]. Koo, A.J. Metabolism of the plant hormone jasmonate: A sentinel for tissue damage and master regulator of stress response Phytochem. Rev. 17, 51-80 (2018).
[5]. Kramell, R., Miersch, O., Hause, B., et al. Amino acid conjugates of jasmonic acid induce jasmonate-responsive gene expression in barley (Hordeum vulgare L.) leaves FEBS Lett. 414(2), 197-202 (1997).
| Cas No. | 120330-93-0 | SDF | |
| Synonymes | (±)-JA-Ile | ||
| Chemical Name | N-[2-[(1R,2R)-3-oxo-2-(2Z)-2-penten-1-ylcyclopentyl]acetyl]-L-isoleucine, N-[2-[(1S,2S)-3-oxo-2-(2Z)-2-penten-1-ylcyclopentyl]acetyl]-L-isoleucine | ||
| Canonical SMILES | C[C@@H](CC)[C@@H](C(O)=O)NC(C[C@H]([C@H]1C/C=C\CC)CCC1=O)=O | ||
| Formula | C18H29NO4 | M.Wt | 323.4 |
| Solubility | 30mg/mL in ethanol, 16mg/mL in DMSO, 25mg/mL in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0921 mL | 15.4607 mL | 30.9215 mL |
| 5 mM | 0.6184 mL | 3.0921 mL | 6.1843 mL |
| 10 mM | 0.3092 mL | 1.5461 mL | 3.0921 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 2 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *