L-NMMA acetate (Synonyms: LNGmonomethyl Arginine acetate) |
| Catalog No.GC16926 |
L'acétate de L-NMMA est un inhibiteur de l'oxyde nitrique synthase de toutes les isoformes de NOS, y compris NOS1, NOS2 et NOS3.
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Cas No.: 53308-83-1
Sample solution is provided at 25 µL, 10mM.
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L-NMMA acetate is a nitric oxide synthase inhibitor of all NOS isoforms including NOS1, NOS2, and NOS3. The Ki values for nNOS (rat), eNOS (human), and iNOS (mouse) are approximately 0.18, 0.4, and 6 µM, respectively.
L-NMMA, starting from 100 μM, produces a concentration-dependent inhibition of the evoked relaxations (2Hz); maximal inhibition at 1 mM averaged about 35%. The inhibitory effect of L-NMMA is unchanged by previous incubation with D-arginine while it is prevented by L-arginine (L-Arg). L-NMMA does not affect isoprenaline-induced relaxation[2]. Superfusion of L-NMMA reduces arteriolar diameter and causes dose-dependent increases in arteriolar tone. The onset of action of L-NMMA is nearly immediate. L-NMMA inhibits vasodilator responses to the endothelium-dependent vasodilator ACh but not to the endothelium-independent NP. NE induced dose-related vasoconstriction that is significantly potentiated by L-NMMA[3].
References:
[1]. Frey C, et al. L-thiocitrulline. A stereospecific, heme-binding inhibitor of nitric-oxide synthases. J Biol Chem. 1994 Oct 21;269(42):26083-91.
[2]. Maggi CA, et al. Effect of NG-monomethyl L-arginine (L-NMMA) and NG-nitro L-arginine (L-NOARG) on non-adrenergic non-cholinergic relaxation in the circular muscle of the human ileum. Br J Pharmacol. 1991 Aug;103(4):1970-2.
[3]. Nakamura T, et al. Effect of NG-monomethyl-L-arginine on arcade arterioles of rat spinotrapezius muscles. Am J Physiol. 1991 Jul;261(1 Pt 2):H46-52.
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Kinase experiment: |
NOS activity is determined by monitoring the conversion of L-[14C]arginine to [14C]citrulline. For nNOS, reaction mixtures contain, in a final volume of 200 μL, 20 mM Na+ HEPES buffer, pH 7.5, 100 μM EDTA, 2 mM CaCl2, 10 μg/mL calmodulin, 500 μM dithiothreitol, 100 μM THB, 25 μM FAD, 25 μM FMN, 100 μg/mL bovine serum albumin, 20 μM L-[14C]citrulline, 500 PM NADPH, and enzyme. Reaction is initiated by the addition of L-[14C]citrulline; reaction temperature is 25°C. At appropriate times, typically 3.5, 7, and 10.5 min, 50-1.11 portions are removed and added to 200 pl of a stopping solution of 100 mM Na+ HEPES buffer, pH 5.5, containing 5 mM EGTA. Those samples are immediately heated for 1 min in a boiling water bath, chilled, and centrifuged. A portion (225 μL) of the supernatant is fractionated on small Dowex 50 columns. [14C]citrulline is eluted with 2 ml of water and is quantitated by liquid scintillation counting[1]. |
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References: [1]. Frey C, et al. L-thiocitrulline. A stereospecific, heme-binding inhibitor of nitric-oxide synthases. J Biol Chem. 1994 Oct 21;269(42):26083-91. |
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| Cas No. | 53308-83-1 | SDF | |
| Synonymes | LNGmonomethyl Arginine acetate | ||
| Chemical Name | (S,E)-2-amino-5-(2-methylguanidino)pentanoic acid compound with acetic acid (1:1) | ||
| Canonical SMILES | OC([C@H](CCCN/C(N)=N/C)N)=O.OC(C)=O | ||
| Formula | C7H16N4O2.CH3CO2H | M.Wt | 248.28 |
| Solubility | DMSO: .25 mg/ml,Ethanol: 1 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.0277 mL | 20.1386 mL | 40.2771 mL |
| 5 mM | 0.8055 mL | 4.0277 mL | 8.0554 mL |
| 10 mM | 0.4028 mL | 2.0139 mL | 4.0277 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 15 reference(s) in Google Scholar.)
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