ML 171 (Synonyms: 2-APT, ML-171, NSC 57951, NSC 169669) |
Catalog No.GC12291 |
Le ML 171 (2-acétylphénothiazine;2-APT) est un inhibiteur puissant et sélectif de la NADPH oxydase 1 (Nox1) qui bloque la génération de ROS dépendante de Nox1, avec une IC50 de 0,25 μM dans le test de confirmation HEK293-Nox1.
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Cas No.: 6631-94-3
Sample solution is provided at 25 µL, 10mM.
ML171 (2-Acetylphenothiazine;2-APT) is a potent and selective NADPH oxidase 1 (Nox1) inhibitor that blocks Nox1-dependent ROS generation, with an IC50 of 0.25 μM in HEK293-Nox1 confirmatory assay.
Nox1-dependent ROS generation has been shown to play a pivotal role in cell signaling, cell growth, angiogenesis, motility and blood pressure regulation. ML171 strongly blocks ROS generation in HT29 cells (IC50=0.129 μM) and only increasing over-expression of Nox1 can overcome the blockage of ROS generation caused by ML171 treatment in HEK293 cell system reconstituted with all the components required Nox1-dependent ROS generation. ML171 efficiently blocks ROS production measured by carboxy-H2-DCFDA staining as well as DPI used as a positive control. When ML171 is tested in HEK293-Nox1 reconstituted cell system, higher potency in blocking Nox1-dependent ROS generation is observed compared with the parental compound[1].
Reference:
[1]. Gianni D, et al. A novel and specific NADPH oxidase-1 (Nox1) small-molecule inhibitor blocks the formation of functional invadopodia in human colon cancer cells. ACS Chem Biol. 2010 Oct 15;5(10):981-93.
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