Norharmane (Synonyms: 2-Azacarbazole,β-Carboline,2,9-Diazafluorene,NSC 84417) |
| Catalog No.GC15833 |
Norharmane (Norharman), un alcaloÏde β-carboline, est un inhibiteur puissant et réversible de la monoamine oxydase, avec des valeurs IC50 de 6,5 et 4,7 μM pour MAO-A et MAO-B, respectivement.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 244-63-3
Sample solution is provided at 25 µL, 10mM.
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Norharmane acts as a co-mutagen. Norharmane is a natural β-carboline first isolated from plants of the Zygophyllaceae family. It is a heterocyclic amine (HCA) that may also be found in fried meats, tobacco smoke, and coffee. Harman and norharmane are aminocarbolines known as “co-mutagens” because they do not show mutagenicity to Salmonella serovar typhimurium [1][2][3].
Norharmane acts as a co-mutagen. Norharmane is widely distributed in our environment. It was not mutagenic to Salmonella typhimurium TA98 and TA100 either with or without S9 mix, but became mutagenic to S.typhimurium TA98 with S9 mix when non-mutagenic aromatic amines like aniline or o- or m-toluidine are added. Also, Norharmane and aromatic amines induced the formation of DNA adduct in S.typhimurium TA98 [3].
Harman and norharmane with concentrations of 0.275 uM and 0.9 uM respectively might inhibited MAO-A and MAO-B. In adult male rats, a novel cocktail of cigarette smoke constituents, containing five minor alkaloids (nornicotine, cotinine, myosmine, anatabine, and anabasine), two β-carbolines (harman and norharmane), and acetaldehyde, did not significantly enhance nicotine self-administration [2].
References:
[1]. Jamali MA, Zhang Y, Teng H, et al. Inhibitory Effect of Rosa rugosa Tea Extract on the Formation of Heterocyclic Amines in Meat Patties at Different Temperatures. Molecules. 2016 Jan 30;21(2):173.
[2]. Smith TT, Schaff MB, Rupprecht LE, et al. Effects of MAO inhibition and a combination of minor alkaloids, β-carbolines, and acetaldehyde on nicotine self-administration in adult male rats. Drug Alcohol Depend. 2015 Oct 1;155:243-52.
[3]. Mori M1, Totsuka Y, Fukutome K, et al. Formation of DNA adducts by the co-mutagen norharman with aromatic amines. Carcinogenesis. 1996 Jul;17(7):1499-503.
| Cas No. | 244-63-3 | SDF | |
| Synonymes | 2-Azacarbazole,β-Carboline,2,9-Diazafluorene,NSC 84417 | ||
| Chemical Name | 9H-Pyrido[3,4-b]indole | ||
| Canonical SMILES | C1(C=CC=C2)=C2C(C=CN=C3)=C3N1 | ||
| Formula | C11H8N2 | M.Wt | 168.2 |
| Solubility | ≤1mg/ml in ethanol;1mg/ml in DMSO;1mg/ml in dimethyl formamide | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.9453 mL | 29.7265 mL | 59.453 mL |
| 5 mM | 1.1891 mL | 5.9453 mL | 11.8906 mL |
| 10 mM | 0.5945 mL | 2.9727 mL | 5.9453 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 37 reference(s) in Google Scholar.)
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