Nonactin |
Catalog No.GC12090 |
La nonactine est un antibiotique macrotétrolide naturel de Streptomyces griseus.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 6833-84-7
Sample solution is provided at 25 µL, 10mM.
Nonactin, monactin, and dinactin mixture is a macrotetralide antibiotic.[1] This product is a mixture of the macrotetralide antibiotic homologs nonactin, monactin, and dinactin that all act as ionophores for monovalent cations.[2],[3],[4],[5],[6],[7] Nonactin is a mitochondrial membrane uncoupler and an inhibitor of adenine nucleotide translocase (ANT; IC50s = 4.4, 3.3, 4.7, and 3.3 µM for recombinant human ANT1-4, respectively).[8],[9] It selectively induces apoptosis in cancer cells and induces tumor regression in mouse xenograft models expressing mutant β-catenin over cancer cells and xenografts expressing wild-type β-catenin.8 Monactin induces swelling of rat liver mitochondria in medium containing either potassium or sodium, stimulates respiration, and uncouples oxidative phosphorylation.[10] It also inhibits Wnt signaling via inhibition of TCF-β-catenin transcriptional activity.[11] Dinactin inhibits T cell proliferation and cytokine production in vitro and reduces pulmonary eosinophilia in antigen-challenged mice.[12]
Reference:
[1]. Pampaloni, F., Mayer, B., Kabat Vel-Job, K., et al. A novel cellular spheroid-based autophagy screen applying live fluorescence microscopy identifies nonactin as a strong inducer of autophagosomal turnover. SLAS Discov. 22(5), 558-570 (2017).
[2]. Hall, J.E., and Latorre, R. Nonactin-K+ complex as a probe for membrane asymmetry. Biophys.J. 16(1), 99-103 (1976).
[3]. Browne, A., and O'Donnell, M.J. Ammonium secretion by Malpighian tubules of Drosophila melanogaster: Application of a novel ammonium-selective microelectrode. J.Exp.Biol. 216(Pt 20), 3818-3827 (2013).
[4]. Skulskii, I.A., Saris, N.-E.L., Savina, M.V., et al. Uptake of thallous ions by mitochondria is stimulated by nonactin but not by respiration alone. Eur.J.Biochem. 120(2), 263-266 (1981).
[5]. Beck, J., Gerlach, H., Prelog, V., et al. Metabolic products of actinomycetes. XXXV. Constitution of the macrotetrolides, monactin, dinactin, and trinactin. Helvetica Chimica Acta 45, 620-630 (1962).
[6]. Harold, F.M., and Baarda, J.R. Effects of nigericin and monactin on cation permeability of Streptococcus faecalis and metabolic capacities of potassium-depleted cells. J. Bacteriol. 95(3), 816-823 (1968).
[7]. Laprade, R., Grenier, F., Pagé-Dansereau, M., et al. Carrier-mediated ion transport in lipid bilayer membranes. Can. J. Biochem. Cell Biol. 62(8), 738-751 (1984).
[8]. Shikata, Y., Kiga, M., Futamura, Y., et al. Mitochondrial uncoupler exerts a synthetic lethal effect against β-catenin mutant tumor cells. Cancer Sci. 108(4), 772-784 (2017).
[9]. Zhang, Y., Tian, D., Matsuyama, H., et al. Human adenine nucleotide translocase (ANT) modulators identified by high-throughput screening of transgenic yeast. J. Biomol. Screen. 21(4), 381-390 (2016).
[10]. Graven, S.N., Lardy, H.A., and Estrada-O, S. Antibiotics as tools for metabolic studies. 8. Effect of nonactin homologs on alkali metal cation transport and rate of respiration in mitochondria. Biochem. 6(2), 365-371 (1967).
[11]. Tamai, Y., Toume, K., Arai, M.A., et al. Nonactin and related compounds found in a screening program for Wnt signal inhibitory activity. Heterocycles 84(2), 1245-1250 (2012).
[12]. Umland, S.P., Shah, H., Jakway, J.P., et al. Effects of cyclosporin A and dinactin on T-cell proliferation, interleukin-5 production, and murine pulmonary inflammation. Am. J. Respir. Cell Mol. Biol. 20(3), 481-492 (1999).
[1]. Pampaloni, F., Mayer, B., Kabat Vel-Job, K., et al. A novel cellular spheroid-based autophagy screen applying live fluorescence microscopy identifies nonactin as a strong inducer of autophagosomal turnover. SLAS Discov. 22(5), 558-570 (2017).
[2]. Hall, J.E., and Latorre, R. Nonactin-K+ complex as a probe for membrane asymmetry. Biophys.J. 16(1), 99-103 (1976).
[3]. Browne, A., and O'Donnell, M.J. Ammonium secretion by Malpighian tubules of Drosophila melanogaster: Application of a novel ammonium-selective microelectrode. J.Exp.Biol. 216(Pt 20), 3818-3827 (2013).
[4]. Skulskii, I.A., Saris, N.-E.L., Savina, M.V., et al. Uptake of thallous ions by mitochondria is stimulated by nonactin but not by respiration alone. Eur.J.Biochem. 120(2), 263-266 (1981).
[5]. Beck, J., Gerlach, H., Prelog, V., et al. Metabolic products of actinomycetes. XXXV. Constitution of the macrotetrolides, monactin, dinactin, and trinactin. Helvetica Chimica Acta 45, 620-630 (1962).
[6]. Harold, F.M., and Baarda, J.R. Effects of nigericin and monactin on cation permeability of Streptococcus faecalis and metabolic capacities of potassium-depleted cells. J. Bacteriol. 95(3), 816-823 (1968).
[7]. Laprade, R., Grenier, F., Pagé-Dansereau, M., et al. Carrier-mediated ion transport in lipid bilayer membranes. Can. J. Biochem. Cell Biol. 62(8), 738-751 (1984).
[8]. Shikata, Y., Kiga, M., Futamura, Y., et al. Mitochondrial uncoupler exerts a synthetic lethal effect against β-catenin mutant tumor cells. Cancer Sci. 108(4), 772-784 (2017).
[9]. Zhang, Y., Tian, D., Matsuyama, H., et al. Human adenine nucleotide translocase (ANT) modulators identified by high-throughput screening of transgenic yeast. J. Biomol. Screen. 21(4), 381-390 (2016).
[10]. Graven, S.N., Lardy, H.A., and Estrada-O, S. Antibiotics as tools for metabolic studies. 8. Effect of nonactin homologs on alkali metal cation transport and rate of respiration in mitochondria. Biochem. 6(2), 365-371 (1967).
[11]. Tamai, Y., Toume, K., Arai, M.A., et al. Nonactin and related compounds found in a screening program for Wnt signal inhibitory activity. Heterocycles 84(2), 1245-1250 (2012).
[12]. Umland, S.P., Shah, H., Jakway, J.P., et al. Effects of cyclosporin A and dinactin on T-cell proliferation, interleukin-5 production, and murine pulmonary inflammation. Am. J. Respir. Cell Mol. Biol. 20(3), 481-492 (1999).
Review for Nonactin
Average Rating: 5
(Based on Reviews and 40 reference(s) in Google Scholar.)Review for Nonactin
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *