Rhodamine 123 (chloride) (Synonyms: R-22420,R-302,RH123) |
Catalog No.GC15795 |
La rhodamine 123 (chlorure) (RH-123; R-22420) est un colorant fluorescent (Λex=503 nm, Λem=527 nm).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 62669-70-9
Sample solution is provided at 25 µL, 10mM.
Rhodamine 123 (Rh123) is a cell-permeable cationic fluorescent probe that specifically recognizes mitochondrial membrane potential and thus accumulates in mitochondria. Rhodamine 123 incorporated into cells is preferentially retained in mitochondria, producing bright fluorescence with fluorescence intensity proportional to mitochondrial membrane potential. Loss of mitochondrial integrity or excessive opening of the mitochondrial permeability transition pore can cause the probe to leak out of the mitochondria, resulting in reduced fluorescence[1]. Rhodamine dye has low toxicity to cells at a certain concentration, so it is often used to detect mitochondria in animal cells, plant cells, and microorganisms[2].
References:
[1]. Cristiano Ferlini, Giovanni Scambia. Assay for apoptosis using the mitochondrial probes, Rhodamine123 and 10-N-nonyl acridine orange. 2007;2(12):3111-4. doi: 10.1038/nprot.2007.397.
[2]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448.
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