Ro 08-2750 |
| Catalog No.GC17622 |
Ro 08-2750 est un inhibiteur non peptidique et réversible du facteur de croissance nerveuse (NGF) qui se lie au NGF et avec une CI50 d'environ 1 μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 37854-59-4
Sample solution is provided at 25 µL, 10mM.
Ro 08-2750 is a p75 neurotrophin receptor (p75NTR) ligand that completely inhibits nerve growth factor (NGF) binding to p75NTR in PC12 cells when used at a concentration of 100 μM.[1] It also binds to NGF dimers (Kd = ~1 μM) to induce a conformational change that prevents NGF binding to p75NTR or tropomyosin-related kinase A (TrkA). Ro 08-2750 inhibits NGF-induced death of SK-N-MC cells and inhibits autophosphorylation of TrkA in PC12 cells. It reverses NGF-induced decreases in β-catenin mRNA expression in, and migration of, SKOV3 and OVCAR-3 ovarian cancer cells.[2]
Reference:
[1]. Niederhauser, O., Mangold, M., Schubenel, R., et al. NGF ligand alters NGF signaling via p75NTR and trkA. J. Neurosci. Res. 61(3), 263-272 (2000).
[2]. Li, B., Cai, S., Zhao, Y., et al. Nerve growth factor modulates the tumor cells migration in ovarian cancer through the WNT/β-catenin pathway. Oncotarget 7(49), 81026-81048 (2016).
| Cas No. | 37854-59-4 | SDF | |
| Chemical Name | 7,10-dimethyl-2,4-dioxo-2,3,4,10-tetrahydrobenzo[g]pteridine-8-carbaldehyde | ||
| Canonical SMILES | O=C1NC(N=C2N(C)C3=CC(C=O)=C(C)C=C3N=C21)=O | ||
| Formula | C13H10N4O3 | M.Wt | 270.24 |
| Solubility | DMSO : 4 mg/mL (14.80 mM; ultrasonic and warming and heat to 80°C) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.7004 mL | 18.5021 mL | 37.0041 mL |
| 5 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL |
| 10 mM | 0.37 mL | 1.8502 mL | 3.7004 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 27 reference(s) in Google Scholar.)
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