Z-DEVD-FMK |
Catalog No.GC12287 |
Z-DEVD-FMK is a specific irreversible cysteine-aspartic protease 3 (caspase-3) inhibitor with an IC50 of 18μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 210344-95-9
Sample solution is provided at 25 µL, 10mM.
Z-DEVD-FMK is a specific irreversible cysteine-aspartic protease 3 (caspase-3) inhibitor with an IC50 of 18μM[1]. Z-DEVD-FMK is commonly used to study apoptosis and neuroprotection [2].
In vitro, Z-DEVD-FMK (50μM) treated cardiomyocytes for 24h, significantly reduced ceramide-induced apoptosis and increased cell survival rate to 81% [3]. Z-DEVD-FMK (15μM) can effectively reduce caspase-3 activity when treating renal proximal tubular cells, but the effect of reducing cell apoptosis is not as good as that of pan-caspase inhibitors [4].
In vivo, Z-DEVD-FMK (160 ng) treated mice with traumatic brain injury (TBI) via intracerebroventricular injection, reduced the lesion volume after central nervous system injury, and improved motor and cognitive functions [2]. Z-DEVD-FMK (20 mg/kg) treated diabetic mice via intraperitoneal injection for 8 weeks, which improved proteinuria, renal function, and tubulointerstitial fibrosis in mice, and slowed down the decrease in serum albumin levels [5] . Z-DEVD-FMK (0.2 mg/kg) is injected into the eyeball to treat optic nerve injury in rabbits, significantly reducing the apoptosis of retinal ganglion cells and promoting the recovery of optic nerve function [6].
References:
[1] Kanthasamy A G, Anantharam V, Zhang D, et al. A novel peptide inhibitor targeted to caspase-3 cleavage site of a proapoptotic kinase protein kinase C delta (PKCδ) protects against dopaminergic neuronal degeneration in Parkinson’s disease models[J]. Free Radical Biology and Medicine, 2006, 41(10): 1578-1589.
[2] Knoblach S M, Alroy D A, Nikolaeva M, et al. Caspase inhibitor z-DEVD-fmk attenuates calpain and necrotic cell death in vitro and after traumatic brain injury[J]. Journal of Cerebral Blood Flow & Metabolism, 2004, 24(10): 1119-1132.
[3] Wang J, Zhen L, Klug M G, et al. Involvement of caspase 3-and 8-like proteases in ceramide-induced apoptosis of cardiomyocytes[J]. Journal of cardiac failure, 2000, 6(3): 243-249.
[4] Yang B, El Nahas A M, Fisher M, et al. Inhibitors directed towards caspase-1 and-3 are less effective than pan caspase inhibition in preventing renal proximal tubular cell apoptosis[J]. Nephron Experimental Nephrology, 2004, 96(2): e39-e51.
[5] Wen S, Wang Z H, Zhang C X, et al. Caspase-3 promotes diabetic kidney disease through gasdermin E-mediated progression to secondary necrosis during apoptosis[J]. Diabetes, Metabolic Syndrome and Obesity, 2020: 313-323.
[6] Zhang W, Yu J G, Wang X, et al. Experimental study on treatment of rabbits optic nerve injury with Caspase-3 inhibitor z-DEVD-fmk[J]. [Zhonghua yan ke za Zhi] Chinese Journal of Ophthalmology, 2010, 46(12): 1084-1089.
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