Substance P 7-11 |
| Catalog No.GC37698 |
Substance P 7-11 is a C-terminal fragment of Substance P which can cause an increase in the intracellular calcium concentration.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 51165-05-0
Sample solution is provided at 25 µL, 10mM.
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Substance P (7-11) is a C-terminal fragment of Substance P which can cause an increase in the intracellular calcium concentration.
Substance P (7-11) increases PGE2 and collagenase production at concentrations greater than 1 μM. Substance P (7-11), but not intact SP, SP-(1-4), SP-(1-6), SP- (8-11) or SP-(9-1 1), nor the tachykinins NKA and NKB, causes an increase in the intracellular calcium concentration as measured by the fluorescent dye Fura-2. The maximal change in intracellular calcium induced by 10 μM Substance P (7-11) was 140±30 nM[1].
[1]. Halliday DA, et al. The substance P fragment SP-(7-11) increases prostaglandin E2, intracellular Ca2+ and collagenase production in bovine articular chondrocytes. Biochem J. 1993 May 15;292 ( Pt 1):57-62.
Cell experiment: | Confluent chondrocytes are incubated for 24 h with SP or Substance P (7-11). TIMP activity is inactivated following reduction and alkylation with 2 mM dithiothreitol and 5 mM iodoacetamide for 30 min at 37°C. Samples are subsequently dialysed. Latent collagenase is activated with 1 mM APt followed by inactivation with soy bean trypsin inhibitor. Collagenase is measured[1]. |
References: [1]. Halliday DA, et al. The substance P fragment SP-(7-11) increases prostaglandin E2, intracellular Ca2+ and collagenase production in bovine articular chondrocytes. Biochem J. 1993 May 15;292 ( Pt 1):57-62. | |
| Cas No. | 51165-05-0 | SDF | |
| Formula | C31H44N6O5S | M.Wt | 612.78 |
| Solubility | H2O : 1.96 mg/mL (3.20 mM; ultrasonic and adjust pH to 2 with HCl) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6319 mL | 8.1595 mL | 16.3191 mL |
| 5 mM | 0.3264 mL | 1.6319 mL | 3.2638 mL |
| 10 mM | 0.1632 mL | 0.816 mL | 1.6319 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
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Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)
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