Crotonoside (Synonyms: 2-Hydroxyadenosine, Isoguanine riboside, Isoguanosine, NSC 12161) |
| Catalog No.GC38412 |
Crotonoside is a FLT3 and HDAC3/6 inhibitor that can be used to study acute myeloid leukemia (AML).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1818-71-9
Sample solution is provided at 25 µL, 10mM.
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Crotonoside is a FLT3 and HDAC3/6 inhibitor that can be used to study acute myeloid leukemia (AML)[1].
Crotonoside (50μM, 24h) induced late apoptosis in about 50% AML cell line MV4-11[1]. Crotonoside (7.5, 15, or 30μM; 48h) significantly induced AML cell apoptosis in a dose-dependent manner[2].
Crotonoside (25, 50, or 100mg/kg; 20 days; i.p.) ameliorated the severity of collagen-induced arthritis (CIA) in mice[3]. Compared with the rabbits in the normal saline (NS) group, the group given Crotonoside (4.5mg/kg, intravenous injection) effectively prolonged the time of aconitine-induced arrhythmias and significantly reduced the incidence of ventricular tachycardia (VT) and ventricular fibrillation (VF)[4].
References:
[1] Li YZ, Yu S, Yan PA, et al. Crotonoside exhibits selective post-inhibition effect in AML cells via inhibition of FLT3 and HDAC3/6. Oncotarget. 2017 Nov 11;8(61):103087.
[2] Ma C, Liu P, Cui S, et al. The identification of APOBEC3G as a potential prognostic biomarker in acute myeloid leukemia and a possible drug target for crotonoside. Molecules. 2022 Sep 7;27(18):5804.
[3] Lin SC, Lin CC, Li S, et al. Alleviation of collagen-induced arthritis by crotonoside through modulation of dendritic cell differentiation and activation. Plants. 2020 Nov 10;9(11):1535.
[4] Liu Z, Jia Y, Song L, et al. Antiarrhythmic effect of crotonoside by regulating sodium and calcium channels in rabbit ventricular myocytes. Life sciences. 2020 Mar 1;244:117333.
| Cell experiment [1]: | |
Cell lines | Acute myeloid leukemia (AML) cell line MV4-11 |
Preparation Method | MV4-11 cells were treated with Crotonoside (25, 50, and 100μM) or 0.1% DMSO for 24 hours. After treatment MV4-11 cells were harvested, washed with PBS and stained for apoptosis using the Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit. |
Reaction Conditions | 25, 50, and 100μM; 16 or 24 hours |
Applications | Crotonoside (50μM, 24h) induced late apoptosis in about 50% AML cells. |
| Animal experiment [2]: | |
Animal models | Collagen-induced arthritic (CIA) mouse model |
Preparation Method | Crotonoside was dissolved in a solvent of 10% DMSO and 90% glyceryl trioctanoate. The mice were randomly divided into four groups (n = 6 per group), including normal, CIA vehicle control, Methotrexate (MTX)-treated and Crotonoside-treated mice at 25, 50, or 100mg/kg. MTX (0.5mg/kg) and Crotonoside treatments were intraperitoneally administered once daily from day 21 to day 41 after the first immunization, whereas vehicle control mice received the solvent. |
Dosage form | 25, 50, or 100mg/kg; 20 days; i.p. |
Applications | Crotonoside ameliorated the severity of collagen-induced arthritis (CIA) in mice. |
References: | |
| Cas No. | 1818-71-9 | SDF | |
| Synonyms | 2-Hydroxyadenosine, Isoguanine riboside, Isoguanosine, NSC 12161 | ||
| Canonical SMILES | OC[C@@H]1[C@H]([C@H]([C@H](N2C=NC(C(N)=N3)=C2NC3=O)O1)O)O | ||
| Formula | C10H13N5O5 | M.Wt | 283.24 |
| Solubility | DMSO: 25 mg/mL (88.26 mM) | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.5306 mL | 17.6529 mL | 35.3057 mL |
| 5 mM | 0.7061 mL | 3.5306 mL | 7.0611 mL |
| 10 mM | 0.3531 mL | 1.7653 mL | 3.5306 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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