CleanCap Reagent GG |
Catalog No.GN20151 |
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
CleanCap Reagent GG is designed for the cotranscriptional capping of mRNA to produce an mRNA with naturally occurring Cap 1. Cap 1 mRNAs have superior in vivo activity compared to Cap 0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA).
CleanCap can be used in conjunction with GlpBio's catalog of modified NTPs.
Using the recommended conditions, transcription with CleanCap GG results in 70-90% capped material, generating a Cap 1 structure and gives crude yields of ~1.5 mg per mL of transcription. Final capping is dependent upon the DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition can affect final capping efficiency.
purity | >95.00% | Extinction Coefficient | 31,700 Lmol-1cm-1 at 260 nm |
Formula | C32H45N15O25P4 (free acid) | M.Wt | 1175.7 g/mole (free acid) |
Salt Form | Na+ | Concentration | 50 mM |
Buffer | H2O | Storage | -20°C or below |
Synonyms | CleanCap Reagent GG for co-transcriptional capping of mRNA, m7G(5')ppp(5')(2'OMeG)pG | Backbone | 5'-5'-Triphosphate |
Base Analog | Guanosine | Sugar Type | RNA |
Nucleotide Category | Cap analogs | ||
Applications | CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development |
Average Rating: 5
(Based on Reviews and 18 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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