IraZolve-Mito (Synonyms: C36H26IrN8 • F6P) |
| Catalog No.GC43908 |
IraZolve-Mito is a fluorescent probe that can be used to label mitochondria in live cells and tissue sections.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2172800-69-8
Sample solution is provided at 25 µL, 10mM.
IraZolve-Mito is a fluorescent probe that can be used to label mitochondria in live cells and tissue sections. It can easily penetrate the cell membrane and accumulate in mitochondria, and its fluorescence can be evaluated for mitochondrial structure using fluorescence microscopy, confocal microscopy, and two-photon microscopy. The excitation/emission peaks of IraZolve-Mito are 405/600 nm. It is suitable for live cell and tissue applications.
a. Reconstitute 0.5 mg IraZolve-Mito with 55 μL DMSO to prepare a 10 mM IraZolve-Mito stock solution. b. Store IraZolve-Mito stock solution at 4°C protected from light. Note 1: IraZolve-Mito stock solution should be used within two months of reconstitution for best staining results.
a. Adherent cells: i．Grow cells in 6-well plate on coverslips to desired confluency (~70-80%). ii. Remove culture medium and add pre-warmed PBS (37°C) or serum-free medium containing IraZolve-Mito at a final concentration of 10-50 μM. iii. Incubate cells at 37°C, 5% CO2 for 30 minutes. iv. Wash cells 2 x 1 minute in PBS. v. Mount coverslips in aqueous mounting media. vi. Observe cells using fluorescence technique of choice. Note 2: Glycerol based mounting media may reduce fluorescence intensity of IraZolve-Mitomm b. Suspension cells: i．Pellet cell suspension and remove supernatant. ii. Resuspend cells in pre-warmed PBS (37°C) or serum-free medium containing IraZolve-Mito at a final concentration of 10-50 μM. iii. Incubate cells at 37°C, 5% CO2 for 30 minutes. iv. Re-pellet the cells and resuspend in PBS or serum-free medium. v. Pipette cells onto a coverslip for imaging in PBS or serum-free medium OR adhere cells to a poly-L-lysine (or similar) coated coverslip by pipetting cells onto the coverslip, allow cells to settle for 2-5 minutes, and wet mount coverslip. vi. Observe using fluorescence technique of choice. Note 3: Optimal staining may vary between cell lines. Staining conditions may be modified according to cell type. Note 4: For epifluorescence applications, IraZolve-Mito can be excited at approximately 365 nm (UV) or405 nm. For confocal and two-photon applications, it can be excited at 400 nm and 800-830 nm, respectively.
**Note 5: Tissue can be stained immediately upon collection or stored for later staining. IraZolve-Mito is compatible with tissue preserved using 4% paraformaldehyde fixation and flash freezing. Note 6: To quench endogenous fluorescence, incubate samples in PBS (pH 7.4) with 100 mM glycine for 20 minutes at room temperature. Other methods to quench fluorescence may be used, such as UV irradiation, however, harsh treatments may induce lipid leaching and/or interfere with lipid binding and should be avoided. a. Staining tissue sections: i. Incubate fresh, fixed, or thawed tissue samples with PBS containing IraZolve-Mito at a final concentration of 10-50μM for 30 minutes at room temperature. ii. Wash samples 3 x 5 minutes in PBS. iii. Mount coverslips using aqueous mounting media. iv. Observe using fluorescence technique of choice. This protocol only provides a guideline, and should be modified according to your specific needs. |
| Cas No. | 2172800-69-8 | SDF | |
| Synonyms | C36H26IrN8 • F6P | ||
| Chemical Name | (OC-6-44)-[4-[6-(2-methyl-2H-tetrazol-5-yl-κN4)-3-pyridinyl-κN]benzonitrile]bis[2-(2-pyridinyl-κN)phenyl-κC]-iridium(1+), monohexafluorophosphate(1-) | ||
| Canonical SMILES | CN1N=[N]([Ir+3]23([C-]4=CC=CC=C4C5=CC=CC=[N]25)([C-]6=CC=CC=C6C7=CC=CC=[N]37)[N]8=CC(C9=CC=C(C#N)C=C9)=CC=C%108)C%10=N1.[F-][P+5]([F-])([F-])([F-])([F-])[F-] | ||
| Formula | C36H26IrN8•F6P | M.Wt | 907.8 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1016 mL | 5.5078 mL | 11.0156 mL |
| 5 mM | 0.2203 mL | 1.1016 mL | 2.2031 mL |
| 10 mM | 0.1102 mL | 0.5508 mL | 1.1016 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 31 reference(s) in Google Scholar.)
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