Irisin (human, recombinant) (Synonyms: Fibronectin Type Ill Domain 5; FNDC5; FRCP2; Pep) |
| Catalog No.GB40243 |
Source:Recombinant protein expressed in E. coli. An N-terminal His-tag and SUMOpro tag were removedby cleavage with SUMO protease 1 (Ulp1).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9037-90-5
Sample solution is provided at 25 µL, 10mM.
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Formulation: 50 mM Tris pH 8.0, 150 mM sodium chloride, and 20% glycerol
Amino Acids:1-111 (full-length, amino acids 32-143 of FNDC5)
Physical exertion and exercise are the primary defenses against obesity, insulin resistance, and diabetes. The transcriptional co-activator peroxisome proliferator-activated receptor C coactivator 1α (PGC-1α), regulates mitochondrial biogenesis and function. Expression of the membrane protein Fibronectin type III domain-containing protein 5 (FNDC5) is stimulated in muscle by PGC-1α in response to exercise. FNDC5 is proteolytically cleaved and secreted as the hormone peptide irisin (named after the Greek goddess messenger Iris). Irisin has been shown to convert white adipose fat to brown adipose fat upon physical exertion. Brown adipose fat allows for mitochondrial uncoupling leading to thermogenetic programs and heat expenditure. The physiological responses induced by the effects of irisin have the potential to increase weight loss and reduce insulin resistance and obesity.
| Cas No. | 9037-90-5 | SDF | |
| Synonyms | Fibronectin Type Ill Domain 5; FNDC5; FRCP2; Pep | ||
| Formula | M.Wt | 12.4 kDa | |
| Solubility | Storage | -80°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >85.00% estimated by SDS-PAGE
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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