2-Arachidonoyl Glycerol-d8 (Synonyms: 2AGd8) |
| カタログ番号GC46535 |
2アラキドノイルグリセロールの定量化のための内部標準、強力な内因性CB1作動薬
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 924894-97-3
Sample solution is provided at 25 µL, 10mM.
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2-Arachidonoyl glycerol-d8 (2-AG-d8) is intended for use as an internal standard for the quantification of 2-AG by GC- or LC-MS. 2-AG is an endogenous agonist of the CB1 receptor.1,2 Unlike anandamide, 2-AG is present at relatively high levels in the central nervous system; it is the most abundant molecular species of monoacylglycerol found in rat brain.1,3 Formation of 2-AG is calcium-dependent and is mediated by the activities of phospholipase C and diacylglycerol lipase.1 2-AG acts as a full agonist at the CB1 receptor. At a concentration of 0.3 nM, 2-AG induces a rapid, transient increase in intracellular free calcium in NG108-15 neuroblastoma X glioma cells through a receptor-dependent mechanism.4
1.Stella, N., Schweitzer, P., and Piomelli, D.A second endogenous cannabinoid that modulates long-term potentiationNature388(6644)773-778(1997) 2.Sugiura, T., Kodaka, T., Nakane, S., et al.Evidence that the cannabinoid CB1 receptor is a 2-arachidonoylglycerol receptor. Structure-activity relationship of 2-arachidonoylglycerol, ether-linked analogues, and related compoundsJ. Biol. Chem.274(5)2794-2801(1999) 3.Kondo, S., Kondo, H., Nakane, S., et al.2-Arachidonoylglycerol, an endogenous cannabinoid receptor agonist: Identification as one of the major species of monoacylglycerols in various rat tissues, and evidence for its generation through Ca2+-dependent and -independent mechanismsFEBS Letters429(2)152-156(1998) 4.Sugiura, T., Kodaka, T., Kondo, S., et al.Is the cannabinoid CB1 receptor a 2-arachidonoylglycerol receptor• Structural requirements for triggering a Ca2+ transient in NG108-15 cellsJ. Biochem.122(4)890-895(1997)
| Cas No. | 924894-97-3 | SDF | |
| 同義語 | 2AGd8 | ||
| Canonical SMILES | CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)OC(CO)CO | ||
| Formula | C23H30D8O4 | M.Wt | 386.6 |
| 溶解度 | DMSO: 10 mg/ml,Ethanol: Miscible,PBS (7.2): ~150 μg/ml | Storage | Store at -80°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5867 mL | 12.9333 mL | 25.8665 mL |
| 5 mM | 0.5173 mL | 2.5867 mL | 5.1733 mL |
| 10 mM | 0.2587 mL | 1.2933 mL | 2.5867 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)
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