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Ac-DEVD-AMC (Synonyms: NAcetylAspGluValAsp7amido4Methylcoumarin, Caspase3 Substrate (Fluorogenic))

カタログ番号GC42686

Ac-DEVD-AMC は、カスパーゼ 3 蛍光基質です。

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Ac-DEVD-AMC 化学構造

Cas No.: 169332-61-0

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1mg
$45.00
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5mg
$135.00
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10mg
$209.00
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Sample solution is provided at 25 µL, 10mM.

Description of Ac-DEVD-AMC

Ac-DEVD-AMC is the Caspase-3 substrate.

Ac-DEVD-AMC is the caspase-3 substrate and could be used to monitor intracellular caspase-3 activity[1].The AMC moiety is highest at 2 h (area =5.6±1.8), the AMC peak area for Ac-DEVD-AMC incubated without lung specimen (substrate alone) for 6 h is relatively small[2].

References:
[1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.
[2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.

Protocol of Ac-DEVD-AMC

Cell experiment:

Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2 : 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through microcentrifuge filter, separateed on HPLC, and analyzed for the fluorogenic AMC moiety[2].

References:

[1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.
[2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.

Chemical Properties of Ac-DEVD-AMC

Cas No. 169332-61-0 SDF
同義語 NAcetylAspGluValAsp7amido4Methylcoumarin, Caspase3 Substrate (Fluorogenic)
Canonical SMILES CC(C1=CC=C(C=C1O2)NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H](CC(O)=O)NC(C)=O)=O)CCC(O)=O)=O)C(C)C)=O)CC(O)=O)=O)=CC2=O
Formula C30H37N5O13 M.Wt 675.6
溶解度 DMSO: 10 mg/ml Storage -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Ac-DEVD-AMC

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 1.4802 mL 7.4008 mL 14.8017 mL
5 mM 0.296 mL 1.4802 mL 2.9603 mL
10 mM 0.148 mL 0.7401 mL 1.4802 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.

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