Bromobimane (Monobromobimane) (Synonyms: MBBr, NSC 608544; (mBBr)) |
| カタログ番号GC30043 |
ブロモビマン (モノブロモビマン) は本質的に非蛍光性であり、小さなチオールと反応すると蛍光生成物に変換されます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 71418-44-5
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Bromobimane (Monobromobimane) is a cell-permeable, thiol-reactive fluorescent probe with maximum absorption and emission wavelengths at 398nm and 490nm, respectively[1, 2, 3]. Bromobimane (Monobromobimane) reacts rapidly with available sulfhydryl groups at physiological pH and produces a stable fluorescent signal[4]. Bromobimane (Monobromobimane) can be used to evaluate or quantify a variety of compounds containing reactive sulfur or thiol groups, including H2S, glutathione (GSH), proteins, and nucleotides[5, 6]. Bromobimane (Monobromobimane) can be used to label GSH in liver sections on slides[7].
References:
[1] Maeda K, Hägglund P, Finnie C, et al. Proteomics of disulphide and cysteine oxidoreduction[J]. Annual Plant Reviews Volume 28: Plant Proteomics, 2006: 71-97.
[2] Weber J, Bijol V, Wilke-Mounts S, et al. Cysteine-reactive fluorescence probes of catalytic sites of ATP synthase[J]. Archives of biochemistry and biophysics, 2002, 397(1): 1-10.
[3] Mohsin N Y, Demir H, Hadwan M H, et al. A New Fluorescent Method for Measuring Peroxiredoxin Enzyme Activity Using Monobromobimane[J]. Journal of Fluorescence, 2024: 1-9.
[4] Sardi F, Manta B, Portillo-Ledesma S, et al. Determination of acidity and nucleophilicity in thiols by reaction with monobromobimane and fluorescence detection[J]. Analytical Biochemistry, 2013, 435(1): 74-82.
[5] Głowacki R, Piechocka J, Bald E, et al. Application of separation techniques in analytics of biologically relevant sulfur compounds[M]//Handbook of Bioanalytics. Cham: Springer International Publishing, 2022: 1-24.
[6] Gilbert H T J, Mallikarjun V, Dobre O, et al. Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading[J]. Nature communications, 2019, 10(1): 4149.
[7] Wang H, Zhang R, Bridle K R, et al. Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury[J]. Scientific Reports, 2017, 7(1): 45374.
This plan only provides a guide, please modify it to meet your specific needs.
1. Solution preparation
(1) Stock solution: Dissolve Bromobimane in DMSO or acetonitrile to prepare a stock solution with a concentration of 100mM.
Note: The stock solution should be purged with inert gas. After unused stock solution is aliquoted, store it at -20°C or -80°C in the dark to avoid repeated freezing and thawing.
(2) Working solution: Before the formal experiment, dilute the stock solution with an appropriate buffer (such as serum-free culture medium or PBS) to the required working concentration, such as 400μM.
Note: Please adjust the optimal working concentration according to the actual situation or refer to the literature to set the gradient concentration by yourself. The working solution must be prepared and used immediately.
2. Labeling proteins with Bromobimane[1] (from the literature, for reference only)
(1) Remove the cell culture medium and wash the cells in PBS.
(2) Label the cells by incubating with 2mL 400μM Bromobimane in PBS at 37°C for 10min.
(3) Add 50μL of 0.4M glutathione in PBS to each well to quench the Bromobimane reaction.
(4) Remove the Bromobimane solution and wash the cells with PBS. Incubate with 1mL of trypsin at 37°C for 10min to detach the cells from the substrate.
(5) Neutralize the trypsin activity with serum-containing medium and pellet the cells by centrifugation at 400g for 5min.
(6) Resuspend the cells in cold PBS and pellet the cells by centrifugation at 400g for 5min. Store the cell pellet at -20°C before proteomic analysis.
References:
[1] Gilbert H T J, Mallikarjun V, Dobre O, et al. Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading[J]. Nature communications, 2019, 10(1): 4149.
| Cas No. | 71418-44-5 | SDF | |
| 同義語 | MBBr, NSC 608544; (mBBr) | ||
| Canonical SMILES | O=C1N(C(C(C)=C2C)=O)N2C(CBr)=C1C | ||
| Formula | C10H11BrN2O2 | M.Wt | 271.11 |
| 溶解度 | DMSO : ≥ 86.6 mg/mL (319.43 mM) | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6885 mL | 18.4427 mL | 36.8854 mL |
| 5 mM | 0.7377 mL | 3.6885 mL | 7.3771 mL |
| 10 mM | 0.3689 mL | 1.8443 mL | 3.6885 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 19 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *