BSA Control for Fatty Acid Complexes (5 mM) |
| カタログ番号GC46102 |
BSA-脂肪酸複合体の制御
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: N/A
Sample solution is provided at 25 µL, 10mM.
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BSA control for BSA-fatty acid complexes (5 mM) is a fatty acid-free preparation of BSA intended for use as a control for BSA-fatty acid complexes containing 5 mM fatty acid (Cas No. GC46103). It can be used as a control for experiments using BSA-fatty acid complexes to deliver fatty acids to cells in culture for the purpose of monitoring fatty acid oxidation or similar processes in various cellular metabolic studies.[1,2,3] The BSA/BSA-FAs are acceptable for use with short term cell culture applications (acute treatment to 18 hours), however for long term 25+ hours the product can/should be filter sterilized using a 0.2 µm filter and sterile receptacle without affecting the performance.
Formulation: 0.8 mM BSA in 150 mM sodium chloride, pH 7.4
References:
[1]. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).
[2]. Wang, D., Green, M.F., McDonnell, E., et al. Oxygen flux analysis to understand the biological function of sirtuins. Methods Mol. Biol. 1077, 241-258 (2013).
[3]. Bentebibel, A., Sebastián, D., Herrero, L., et al. Novel effect of C75 on carnitine palmitoyltransferase I activity and palmitate oxidation. Biochemistry 45(14), 4339-4350 (2006).
| Cas No. | N/A | SDF | |
| Canonical SMILES | N/A | ||
| Formula | N/A | M.Wt | N/A |
| 溶解度 | Storage | Store at -20°C, protect from light | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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Average Rating: 5 (Based on Reviews and 40 reference(s) in Google Scholar.)
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