C22 Ceramide (d18:1/22:0) (Synonyms: C22 Ceramide；Cer(d18:1/22:0)；Ceramide (d18:1/22:0)) |
| カタログ番号GC43069 |
C-22セラミドは内因性の生体活性スフィンゴ脂質です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 27888-44-4
Sample solution is provided at 25 µL, 10mM.
C22 Ceramide (d18:1/22:0) is a long-chain ceramide containing behenic acid isolated from the sea sponge Haliclona koremella and is an endogenous bioactive sphingolipid. C22 Ceramide (d18:1/22:0) is widely used to study lipid-mediated signal transduction mechanisms that affect cell homeostasis, apoptosis, and differentiation[1][2]. C22 Ceramide (d18:1/22:0) can also be used as a standard for measuring ceramide concentration in samples by high-performance liquid chromatography/tandem mass spectrometry (LC/MS2) and as a standard for the quantification of sphingolipid metabolites[3][4].
C22 Ceramide (d18:1/22:0) (40nmol; 15min) reduces the propensity of C16-ceramide channel formation in isolated rat liver mitochondria and in liposomes[1]. C22 ceramide (d18:1/22:0) can inhibit the mitochondrial to cytoplasmic stress response induced by hsp-6 RNAi[2]. C22 Ceramide (d18:1/22:0) (0.2µM; 48h) activated miR-199a-3p and inhibited mammalian target of rapamycin(mTOR) level, thus activating the mTOR-ULK1 signaling pathway by promoting the expression of unc-51-like autophagy activating kinase 1 (ULK1), B-cell lymphoma-2 (Bcl-2), Beclin-1, autophagy-related gene 5 (ATG5), and microtubule-associated protein light chain 3 (LC3-II) and degrading p62[5].
References:
[1]. Stiban J, Perera M. Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis[J]. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2015, 1848(2): 561-567.
[2]. Kim H E, Grant A R, Simic M S, et al. Lipid biosynthesis coordinates a mitochondrial-to-cytosolic stress response[J]. Cell, 2016, 166(6): 1539-1552. e16.
[3]. Kauhanen D, Sysi-Aho M, Koistinen K M, et al. Development and validation of a high-throughput LC–MS/MS assay for routine measurement of molecular ceramides[J]. Analytical and bioanalytical chemistry, 2016, 408: 3475-3483.
[4]. Aslan M, Afşar E, Kırımlıoglu E, et al. Antiproliferative effects of thymoquinone in MCF-7 breast and HepG2 liver cancer cells: possible role of ceramide and ER stress[J]. Nutrition and Cancer, 2021, 73(3): 460-472.
[5]. Wan J, Zhang S, Li G, et al. Ceramide Ehux-C22 Targets the miR-199a-3p/mTOR Signaling Pathway to Regulate Melanosomal Autophagy in Mouse B16 Cells[J]. International Journal of Molecular Sciences, 2024, 25(15): 8061.
| Cell experiment [1]: | |
Cell lines | B16 cells |
Preparation Method | B16 cells were cultured with RPMI 1640 medium provided with 10% FBS and 1% PS (5% CO2, 37°C) overnight. Dissolved in DMSO and MTBE (1:1), 2µM C22 Ceramide (d18:1/22:0) was added to the cell. |
Reaction Conditions | 0.2µM; 48h |
Applications | C22 Ceramide (d18:1/22:0) activated miR-199a-3p and inhibited mammalian target of rapamycin(mTOR) level, thus activating the mTOR-ULK1 signaling pathway by promoting the expression of unc-51-like autophagy activating kinase 1 (ULK1), B-cell lymphoma-2 (Bcl-2), Beclin-1, autophagy-related gene 5 (ATG5), and microtubule-associated protein light chain 3 (LC3-II) and degrading p62. |
References: | |
| Cas No. | 27888-44-4 | SDF | |
| 同義語 | C22 Ceramide；Cer(d18:1/22:0)；Ceramide (d18:1/22:0) | ||
| Chemical Name | N-[(1S,2R,3E)-2-hydroxy-1-(hydroxymethyl)-3-heptadecen-1-yl]-docosanamide | ||
| Canonical SMILES | OC[C@H](NC(CCCCCCCCCCCCCCCCCCCCC)=O)[C@H](O)/C=C/CCCCCCCCCCCCC | ||
| Formula | C40H79NO3 | M.Wt | 622.1 |
| 溶解度 | 0.15mg/ml in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.6075 mL | 8.0373 mL | 16.0746 mL |
| 5 mM | 0.3215 mL | 1.6075 mL | 3.2149 mL |
| 10 mM | 0.1607 mL | 0.8037 mL | 1.6075 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 15 reference(s) in Google Scholar.)
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