CL264

Cell lines

Lewis lung carcinoma (LLC),Bone marrow derived macrophages(BMSMs)

Preparation Method

Macrophages were cultivated in the presence or absence of CL264 for 24 h, before tumor cells were added and co-cultured for 42 h. Radiolabeled thymidine was used to detect tumor cell growth and was added to the co-cultures 18 h before cell harvest.

Reaction Conditions

Macrophages,CL264(40ng/ml),LLC co-cultured for 42 h

Applications

CL264 stimulating the BMDMs and it resulted in inhibition of LLC cells, but only when it was used together with IFN-γ.

Animal models

ild-type C57Bl/6J mice,TLR7 Knock out C57Bl/6J mice

Preparation Method

Cancer cells - Murine lung adenocarcinoma LLC-luc cells (1E6 in 100μl PBS/injection) were injected into the flank of recipient mice. Mice then received intra-tumor injections of CL264 at day 0, 3 and 6 after tumor grafting.

Dosage form

40μg/injection, intra-tumor injection

Applications

Intratumoral injection of the TLR7 agonist CL264 induced a pro-tumorigenic effect.CL264 in TLR7 KO mice, similar to that observed in WT mice.These results clearly demonstrate that the pro-tumorigenic effect of CL264 effect is mediated by an effect on TLR7 expressed by malignant (rather than immune) cells.

References:

[1]. Müller E, Christopoulos PF, et al. Toll-Like Receptor Ligands and Interferon-γ Synergize for Induction of Antitumor M1 Macrophages. Front Immunol. 2017 Oct 26;8:1383.

[2]. Dajon M, Iribarren K, et al. Toll like receptor 7 expressed by malignant cells promotes tumor progression and metastasis through the recruitment of myeloid derived suppressor cells. Oncoimmunology. 2018 Oct 11;8(1):e1505174.