Dabcyl-KTSAVLQSGFRKME-Edans TFA |
| カタログ番号GC60119 |
Dabcyl-KTSAVLQSGFRKME-Edans TFA は蛍光ペプチドです。
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Dabcyl-KTSAVLQSGFRKME-Edans TFA is a fluorogenic peptide. Dabcyl-KTSAVLQSGFRKME-Edans TFA is used as the substrate to measure the enzymatic activities of protease forms. Dabcyl-KTSAVLQSGFRKME-Edans TFA has the potential for study 2019-nCoV (COVID-19) infection[1][2][3].
Recombinant 3CLpro is expressed in Pichia pastoris GS115 as a 42 kDa protein that displays a Km of 15 μM with Dabcyl-KTSAVLQSGFRKME-Edans TFA as substrate[1]. The Dabcyl-KTSAVLQSGFRKME-Edans TFA fluorogenic peptide is used as the substrate and the enhanced fluorescence due to cleavage of this substrate catalyzed by the protease is monitored at 538 nm with an excitation wavelength of 355 nm using a fluorescence plate reader[1].
[1]. Thi Thanh Hanh Nguyen, et al.Flavonoid-mediated Inhibition of SARS Coronavirus 3C-like Protease Expressed in Pichia Pastoris. Biotechnol Lett. 2012 May;34(5):831-8. [2]. Chih-Jung Kuo, et al.Engineering a Novel Endopeptidase Based on SARS 3CL(pro). Biotechniques. 2009 Dec;47(6):1029-32. [3]. Cheng-Wei Yang, et al.Novel Small-Molecule Inhibitors of Transmissible Gastroenteritis Virus. Antimicrob Agents Chemother. 2007 Nov;51(11):3924-31.
| Cas No. | SDF | ||
| Formula | M.Wt | ||
| 溶解度 | DMSO : 100 mg/mL (45.55 mM; Need ultrasonic); H2O : 6.67 mg/mL (3.04 mM; Need ultrasonic) | Storage | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)
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