DYKDDDDK Peptide (trifluoroacetate salt) (Synonyms: DYKDDDDK Epitope, DYKDDDDK Octapeptide, DYKDDDDK Tag)

DYKDDDDK peptide is a synthetic octapeptide and fusion tag.^1,2 It can be added to the N- or C-terminus of proteins or inserted into the protein sequence and later cleaved using enteropeptidase. Due to its hydrophilicity, it has a low potential to interfere with protein folding or activity. DYKDDDDK peptide has commonly been used as an epitope tag on recombinant proteins to facilitate their detection and purification by DYKDDDDK-specific antibodies in vitro and in vivo.^1,2,3,4

1.Hopp, T.P., Prickett, K.S., Price, V.L., et al.A short polypeptide marker sequence useful for recombinant protein identification and purificationNat. Biotechnol.61204-1210(1988) 2.Einhauer, A., and Jungbauer, A.The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteinsJ. Biochem. Biophys. Methods49(1-3)455-465(2001) 3.Ichiki, T., Koga, T., Okuno, T., et al.Modulation of leukotriene B4 receptor 1 signaling by receptor for advanced glycation end products (RAGE)FASEB J.30(5)1811-1822(2016) 4.Ikeda, K., Koga, T., Sasaki, F., et al.Generation and characterization of a human-mouse chimeric high-affinity antibody that detects the DYKDDDDK FLAG peptideBiochem. Biophys. Res. Commun.486(4)1077-1082(2017)