Fluorescein-12-UTP, 10mM Sodium Solution |
| カタログ番号GB20111 |
フルオレセイン-12-UTPは、in vitro転写反応におけるRNAラベリングのためにUTPを置換できるフルオレセインのUTPアナログです。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 185843-23-6
Sample solution is provided at 25 µL, 10mM.
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Fluorescein-12-UTP is a fluorescein-labeled uridine triphosphate (UTP) analog that can be used as a substrate catalyzed by T3/T7/SP6 RNA polymerase and reverse transcriptase to replace UTP in in vitro transcription reactions for RNA mark[1]. Labeled RNA can be detected directly by fluorescence or indirectly by using an anti-luciferin horseradish peroxidase (HRP) or alkaline phosphatase (Alkaline Phosphatase, AP) conjugated antibody [2].
References:
[1]. Gilbert Lauter, Iris SÖll, Giselbert Hauptmann. Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain. 2011 Apr 5;6:10. doi: 10.1186/1749-8104-6-10.
[2]. Brent M. Bany, David G. Simmons. Nonradioactive In Situ Hybridization: Optimization for Tissue Sections from Pregnant Uteri and Placenta during the First Half of Pregnancy. 2014, Pages 591-603. https://doi.org/10.1016/B978-0-12-394445-0.00050-3
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