Z-Gly-Pro-AMC |
カタログ番号GA23817 |
Z-Gly-Pro-AMC は蛍光基質です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 68542-93-8
Sample solution is provided at 25 µL, 10mM.
Z-Gly-Pro-AMC functions as a fluorogenic substrate that is susceptible to hydrolysis by prolyl endopeptidase, resulting in the production of intensely fluorescent 7-amido-4-methylcoumarin. (Λex=380 nm, Λem=465 nm) [1-3].
The substrate Z-Gly-Pro-AMC is specifically cleaved by FAPα, which possesses a postprolyl peptidase activity, capable of targeting N-terminal benzyloxycarbonyl (Z)-blocked peptides. This enzymatic action results in the generation of highly fluorescent 7-amido-4-methylcoumarin[4].
References:
[1]. Bracke A, Van Elzen R, et,al. The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma. Clin Chim Acta. 2019 Aug;495:154-160. doi: 10.1016/j.cca.2019.04.063. Epub 2019 Apr 11. PMID: 30981844.
[2]. Fu P, Sun W, et,al. Identification of two isoforms of Pop in the domestic silkworm, Bombyx mori: Cloning, characterization and expression analysis. Gene. 2018 Aug 15;667:101-111. doi: 10.1016/j.gene.2018.05.021. Epub 2018 May 9. PMID: 29753046.
[3]. Lee KN, Jackson KW, et,al. Antiplasmin-cleaving enzyme is a soluble form of fibroblast activation protein. Blood. 2006 Feb 15;107(4):1397-404. doi: 10.1182/blood-2005-08-3452. Epub 2005 Oct 13. PMID: 16223769.
[4].Huang S, Zhang Y, et,al. Toxicological profile and safety pharmacology of a single dose of fibroblast activation protein-α-based doxorubicin prodrug: in-vitro and in-vivo evaluation. Anticancer Drugs. 2018 Mar;29(3):253-261. doi: 10.1097/CAD.0000000000000593. PMID: 29346131.
Average Rating: 5
(Based on Reviews and 32 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *