N6-Methyladenosine-5'-Triphosphate |
| カタログ番号GN20007 |
N6-メチルアデノシン（N6-メチルATP）は、アデノシンの塩基修飾された類似体であり、天然RNA中に少量存在する核酸塩基です（Meyer et al.）。
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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N6-Methyladenosine-5-Triphosphate (N6-methyl ATP; m6ATP) is a base-modified analog of adenosine, identified as a minor nucleoside in natural RNA[1]. N6-Methyladenosine-5’-Triphosphate is a dynamic, reversible, covalent modification of ribonucleotides, commonly found in eukaryotic messenger RNA (mRNA), and plays a crucial regulatory role in RNA splicing, export, stability, and translation[2, 3]. N6-Methyladenosine-5-Triphosphate serves as a substrate for RNA polymerase and is used as a complete substitute for ATP in mRNA modification reactions[4, 5]. Additionally, N6-Methyladenosine-5-Triphosphate is an effective agonist for P2Y purinergic receptors in guinea pigs and Escherichia coli[6]. This product is provided in the form of a lithium salt solution.
References:
[1] Yuan Y, Arneson R, Burke E, et al. Efficient 3′-end tailing of RNA with modified adenosine for nanopore direct total RNA sequencing[J]. bioRxiv, 2024: 2024.02. 24.581884.
[2] Zheng H, Li S, Zhang X, et al. Functional implications of active N6-methyladenosine in plants[J]. Frontiers in Cell and Developmental Biology, 2020, 8: 291.
[3] Anderson S J, Kramer M C, Gosai S J, et al. N6-methyladenosine inhibits local ribonucleolytic cleavage to stabilize mRNAs in Arabidopsis[J]. Cell reports, 2018, 25(5): 1146-1157. e3.
[4] Parr C J C, Wada S, Kotake K, et al. N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells[J]. Nucleic Acids Research, 2020, 48(6): e35-e35.
[5] Potapov V, Fu X, Dai N, et al. Base modifications affecting RNA polymerase and reverse transcriptase fidelity[J]. Nucleic Acids Research, 2018, 46(11): 5753-5763.
[6] Burnstock G, Fischer B, Hoyle C H V, et al. Structure activity relationships for derivatives of adenosine‐5′‐triphosphate as agonists at P2 purinoceptors: Heterogeneity within P2x and P2y subtypes[J]. Drug development research, 1994, 31(3): 206-219.
| purity | >99.00% | Extinction Coefficient | 15,567 Lmol-1cm-1 at 265 nm |
| Formula | C11H18N5O13P3 (free acid) | M.Wt | 521.20 g/mole (free acid) |
| Salt Form | Li+ | 濃度 | 100 mM |
| Buffer | H2O | Storage | -20°C or below |
| 同義語 | N6-Methyl-ATP, m6ATP | Backbone | 5'-Triphosphate |
| Base Analog | Adenosine | Sugar Type | RNA |
| Nucleotide Category | Base Modified RNA | ||
| 応用 | Aptamers, Epigenetics/DNA Damage, In vitro Transcription, Mutagenesis, Photocrosslinking Studies | ||
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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