Sudan Red 7B (Synonyms: C.I. 26050, Ceres Red 7B, Fat Red 7B, Hexatype Carmine B, NSC 326202, Solvent Red 19) |
| カタログ番号GC48625 |
アゾ染料
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 6368-72-5
Sample solution is provided at 25 µL, 10mM.
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Sudan Red 7B is a non-fluorescent, fat-soluble azo dye with a maximum absorption wavelength of 525nm[1, 2]. Sudan Red 7B can be used to stain fat in plant and animal tissues and can also indicate the presence of suberin in cell walls[3, 4]. Sudan Red 7B is also used as a standard for the detection of banned colorants in food[5].
References:
[1] Firoozeh F, Shahamat Y D, Rodríguez-Couto S, et al. Bioremediation for the Decolorization of Textile Dyes by Bacterial Strains Isolated from Dyeing Wastewater[J]. Jordan Journal of Biological Sciences, 2022, 15(2).
[2] Hassan Q M A, Manshad R K H. Optical limiting properties of sudan red B in solution and solid film[J]. Optical and Quantum Electronics, 2015, 47: 297-311.
[3] Mozgová I, Muñoz-Viana R, Hennig L. PRC2 represses hormone-induced somatic embryogenesis in vegetative tissue of Arabidopsis thaliana[J]. PLoS Genetics, 2017, 13(1): e1006562.
[4] Yang C, Zhang X, Wang T, et al. Phenotypic plasticity in the structure of fine adventitious Metasequoia glyptostroboides roots allows adaptation to aquatic and terrestrial environments[J]. Plants, 2019, 8(11): 501.
[5] Pardo O, Yusà V, León N, et al. Development of a method for the analysis of seven banned azo-dyes in chilli and hot chilli food samples by pressurised liquid extraction and liquid chromatography with electrospray ionization-tandem mass spectrometry[J]. Talanta, 2009, 78(1): 178-186.
This plan only provides a guide, please modify it to meet your specific needs.
1. Solution preparation
(1) Working solution: Dissolve Sudan Red 7B in DMF or DMSO, dilute the stock solution with the experimental buffer, and prepare the required working concentration.
Note: Please adjust the optimal working concentration according to the actual situation or refer to the literature to set the gradient concentration by yourself. The working solution must be prepared and used immediately.
2. Use Sudan Red 7B to detect the structure of the endosperm cuticle[1] (Source literature, for reference only)
(1) The samples were fixed overnight in phosphate buffer (pH 7.2, containing 4% formaldehyde and 0.25% glutaraldehyde) at 4°C. At the beginning of fixation, the dry seeds were pierced with a fine needle, and all samples were infiltrated under vacuum. The samples were then embedded in 1.5% agarose particles, dehydrated in a graded ethanol series, and embedded in paraffin 12μm thick sections were cut using a microtome.
(2) Sections were incubated with Sudan Red 7B dissolved in a 1:1 (v/v) mixture of polyethylene glycol 400 and 90% (v/v) glycerol at a final concentration of 0.1% (w/v) for 15min.
(3) Wash the sections with water and mount them in glycerol. Examine the samples under a microscope.
Note: For your safety and health, please wear a lab coat and disposable gloves when operating.
References:
[1]Loubéry S, De Giorgi J, Utz-Pugin A, et al. A maternally deposited endosperm cuticle contributes to the physiological defects of transparent testa seeds[J]. Plant physiology, 2018, 177(3): 1218-1233.
| Cas No. | 6368-72-5 | SDF | |
| 同義語 | C.I. 26050, Ceres Red 7B, Fat Red 7B, Hexatype Carmine B, NSC 326202, Solvent Red 19 | ||
| Canonical SMILES | CCNC(C=CC1=C2C=CC=C1)=C2N=NC(C=C3)=CC=C3N=NC4=CC=CC=C4 | ||
| Formula | C24H21N5 | M.Wt | 379.5 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml,Ethanol: 1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.635 mL | 13.1752 mL | 26.3505 mL |
| 5 mM | 0.527 mL | 2.635 mL | 5.2701 mL |
| 10 mM | 0.2635 mL | 1.3175 mL | 2.635 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 21 reference(s) in Google Scholar.)
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