2-Bromohexadecanoic acid (Synonyms: 2-溴棕榈酸) |
| Catalog No.GC19850 |
2-Bromohexadecanoic acid은 지질 대사의 비선택성 억제제이며 단백질 S-palmitoylation의 일반 억제제이다.
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Cas No.: 18263-25-7
Sample solution is provided at 25 µL, 10mM.
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2-Bromohexadecanoic acid은 지질 대사의 비선택성 억제제이며 단백질 S-palmitoylation의 일반 억제제이다. 2-Bromohexadecanoic acid은 모든 DHHC(Asp-His-His-Cys) 단백질의 팜리토일전전이 효소 활설을 직접적으로 그리고 되돌릴 수 없이 억제합니다[2]. 2-Bromohexadecanoic acid은 쥐 간염 바이러스(MHV-A59)의 복제를 강력하게 억제하고 주로 지질 입자를 특이적으로 파괴함으로써 이를 달성한다[3].
체외 실험에서 Hela 세포에 50μM의 2-Bromohexadecanoic acid을 1시간 미리 처리하여 TNFα+CHX 유도된 세포 피로토시스를 현저하게 억제했고 세포 조직사를 억제하지 않았고 젖산 탈수소효소 석방과 PI-양성 세포를 줄였다[4]. HepG2 세포에 75-150μM의 2-Bromohexadecanoic acid을 처리하여 패리마트(PA) 유도된 암간세포(CSC) 구체 형성을 현저하게 줄였고 세포 활성을 영향을 미치지 않았다[5]. Co-expressing diacylated GAP-43-YFP와 GalNAc-T-CFP를 공표현하는 CHO-K1 세포에 25, 50, 150μM의 2-Bromohexadecanoic acid을 처리하여 성장과 관련된 단백질-43(GAP-43)과 TGN-막의 결합을 현저하게 줄였다[6].
References:
[1] Davda D, El Azzouny M A, Tom C T M B, et al. Profiling targets of the irreversible palmitoylation inhibitor 2-bromopalmitate[J]. ACS chemical biology, 2013, 8(9): 1912-1917.
[2] Yu W, Yang K, Zhao M, et al. Design, synthesis and biological activity evaluation of novel covalent S-acylation inhibitors[J]. Molecular Diversity, 2024, 28(3): 1073-1092.
[3] Liu D, Cruz-cosme R, Leibowitz J, et al. Uncovering the Vital Role of Lipid Droplets in Coronavirus Replication: A Novel Insight Arising from the Biological Activity of 2-Bromopalmitate[J]. bioRxiv, 2023: 2023.06. 29.547086.
[4] Hu L, Chen M, Chen X, et al. Chemotherapy-induced pyroptosis is mediated by BAK/BAX-caspase-3-GSDME pathway and inhibited by 2-bromopalmitate[J]. Cell death & disease, 2020, 11(4): 281.
[5] Chong L W, Tsai C L, Yang K C, et al. Targeting protein palmitoylation decreases palmitate-induced sphere formation of human liver cancer cells[J]. Molecular Medicine Reports, 2020, 22(2): 939-947.
[6] Pedro M P, Vilcaes A A, Tomatis V M, et al. 2-Bromopalmitate reduces protein deacylation by inhibition of acyl-protein thioesterase enzymatic activities[J]. PloS one, 2013, 8(10): e75232.
| 세포 실험 [1]: | |
세포 라인 | Hela 세포 |
제조 방법 | Hela 세포에 50μM의 2-Bromohexadecanoic acid을 1시간 미리 배양한 후 TNFα (20ng/mL) + Cycloheximide (CHX) (10μg/mL)를 지정된 시간 (0, 3, 6, 9, 12시간) 동안 처리한 후 Hoechst 33342/PI 이중 염색을 실시하고 형광 현미경 이미지를 촬영하고 PI-양성 세포 비율을 이미지 J 소프트웨어를 사용하여 결정했습니다. 배양 상청을 수집하여 LDH 방출 비율을 측정했습니다. |
반응 조건 | 50μM; 1시간 동안 |
응용 분야 | 팜리토일레이션 억제제 2-Bromohexadecanoic acid으로 미리 배양된 세포는 TNFα+CHX 처리 후 LDH 릴리즈와 PI 양성 세포를 줄였습니다. |
References: | |
| Cas No. | 18263-25-7 | SDF | |
| Synonyms | 2-溴棕榈酸 | ||
| Formula | CH3(CH2)13CH(Br)CO2H | M.Wt | 335.32 |
| Solubility | Methanol : 100mg/ml(298.2226） | Storage | Store at RT , protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.9822 mL | 14.9111 mL | 29.8223 mL |
| 5 mM | 0.5964 mL | 2.9822 mL | 5.9645 mL |
| 10 mM | 0.2982 mL | 1.4911 mL | 2.9822 mL |
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- Purity: >99.00%
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Average Rating: 5 (Based on Reviews and 19 reference(s) in Google Scholar.)
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