20-HETE-d6 (Synonyms: 20-Hydroxyeicosatetraenoic Acid-d6, 20-hydroxy Arachidonic Acid-d6)

20-HETE-d₆ contains six deuterium atoms at the 16, 16', 17, 17', 18 and 18' positions. It is intended for use as an internal standard for the quantification of 20-HETE by GC- or LC-MS. 20-HETE is a cytochrome P450 (CYP450) metabolite postulated to play an autacoid role in the renal and cerebral vasculature.¹ In rat cerebral microvessels, 20-HETE is a vasoconstrictor that mediates pressure-induced autoregulatory vasoconstriction.² 20-HETE is excreted mainly as the glucuronide conjugate. The concentration of free 20-HETE (20-40 pg/ml in human urine) is about 10-fold lower than the corresponding concentration of the 20-glucuronide.³

1.McGiff, J.C., and Quilley, J.20-HETE and the kidney: Resolution of old problems and new beginningsAm. J. Physiol.277(3 Pt 2)R607-R623(1999) 2.Gebremedhin, D., Lange, A.R., Lowry, T.F., et al.Production of 20-HETE and its role in autoregulation of cerebral blood flowCirc. Res.87(1)60-65(2000) 3.Prakash, C., Zhang, J.Y., Falck, J.R., et al.20-Hydroxyeicosatetraenoic acid is excreted as a glucuronide conjugate in human urineBiochem. Biophys. Res. Commun.185(2)728-733(1992)