3'O-Allyl-ATP, 100mM Sodium Solution |
| Catalog No.GB10784 |
3'O-Allyl-ATP is an allyl-modified ATP analog that is a good substrate for RNA polymerase.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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3'O-Allyl-Adenosine-5'-triphosphate(3'-O-allyladenylic acid,3'O-Allyl-ATP) is a nucleic acid analogue of adenosine acid, which is a good substrate for RNA polymerase. In the structure of 3'O-Allyl-ATP, the 3'-OH group of GTP is replaced by an allyl group, and its incorporation in the synthesis process will cause the termination of the polymerase reaction [1] [2]. Allyl groups can be removed by using catalytic deallylation.
References:
[1]. Jian Wu, et al. 3'-O-modified nucleotides as reversible terminators for pyrosequencing. 2007 Oct 16;104(42):16462-7. doi: 10.1073/pnas.0707495104. Epub 2007 Oct 8.
[2]. Hameer Ruparel, et al.Design and synthesis of a 3'-O-allyl photocleavable fluorescent nucleotide as a reversible terminator for DNA sequencing by synthesis. 2005 Apr 26;102(17):5932-7. doi: 10.1073/pnas.0501962102. Epub 2005 Apr 13.
| purity | ≥98% | Extinction Coefficient | |
| Formula | C13H20N5O13P3(free acid) | M.Wt | 547.25(free acid) |
| Salt Form | Concentration | ||
| Buffer | Storage | -20℃ | |
| Synonyms | 2- amino-adenosine -5'- triphosphate | Backbone | |
| Base Analog | Sugar Type | ||
| Nucleotide Category |
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