Brassinazole |
| Catalog No.GC19544 |
Brassinazole (Brz)은 브라젠노스테로이드(BR) 생합성에서 C-22 히드록시라제(CYP90B1)의 특이성 억제제로, 브라젠노졸의 응용은 BR 결핍 돌연변이를 보충하여 BRs의 기능을 규명하는 효과적인 방법으로 나타났다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 224047-41-0
Sample solution is provided at 25 µL, 10mM.
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Brassinazole (Brz)은 브라젠노스테로이드(BR) 생합성에서 C-22 히드록시라제(CYP90B1)의 특이성 억제제로, 브라젠노졸의 응용은 BR 결핍 돌연변이를 보충하여 BRs의 기능을 규명하는 효과적인 방법으로 나타났다.Brassinazole 은 하이브리드 포플러의 텐션우드 형성 중 G섬유 세포벽 발달과 2차 엑스렘 세포벽 탄수화물 생합성에서 조절 역할을 한다[2]. 또한, Brassinazole 처리(5µM)는 감귤열의 성숙을 지연시키고 호흡 속도를 억제할 수 있다[3].
Brassinazole 처리(5, 1, 0.5μM)는 농도에 따라 아랍디드시앙 유기체의 키를 크게 줄이는 데에 의해 눈에 띄게 작용했다. 또한, Brassinazole 처리(1μM)는 BR 결핍 돌연변이와 유사한 표현형을 보였으며, 이는 브라젠놀라이드(BL)(10nM)의 응용으로 복구될 수 있다. 어둠에서 자란 10일 된 아랍디드시앙 유기체는 0에서 10μM까지의 다양한 농도의 브라지노졸로 처리되었다. Brassinazole 의 농도가 증가함에 따라 하이포코티우스의 길이가 감소하였다. 길이 변화는 0.1에서 0.5μM 사이에서 발생했다[4].
References:
[1]. Oh K, Matsumoto T, Yamagami A, Ogawa A, Yamada K, Suzuki R, et al. YCZ-18 is a new brassinosteroid biosynthesis inhibitor. PLoS One. 2015;10(3):e0120812. Epub 20150320. doi: 10.1371/journal.pone.0120812. PubMed PMID: 25793645.
[2]. Gao J, Yu M, Zhu S, Zhou L, Liu S. Effects of exogenous 24-epibrassinolide and brassinazole on negative gravitropism and tension wood formation in hybrid poplar (Populus deltoids X Populus nigra). Planta. 2019;249(5):1449-63. Epub 20190128. doi: 10.1007/s00425-018-03074-2. PubMed PMID: 30689054.
[3]. He Y, Li J, Ban Q, Han S, Rao J. Role of Brassinosteroids in Persimmon ( Diospyros kaki L.) Fruit Ripening. J Agric Food Chem. 2018;66(11):2637-44. Epub 20180312. doi: 10.1021/acs.jafc.7b06117. PubMed PMID: 29509414.
[4]. Asami T, Min YK, Nagata N, Yamagishi K, Takatsuto S, Fujioka S, et al. Characterization of brassinazole, a triazole-type brassinosteroid biosynthesis inhibitor. Plant Physiol. 2000;123(1):93-100. doi: 10.1104/pp.123.1.93. PubMed PMID.
| Cas No. | 224047-41-0 | SDF | |
| Chemical Name | β-[(4-chlorophenyl)methyl]-α-methyl-α-phenyl-1H-1,2,4-triazole-1-ethanol | ||
| Canonical SMILES | ClC(C=C1)=CC=C1CC(C(C)(O)C2=CC=CC=C2)N3N=CN=C3 | ||
| Formula | C18H18ClN3O | M.Wt | 327.8 |
| Solubility | DMF: 100 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 50 mg/ml,Ethanol: 25 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0506 mL | 15.2532 mL | 30.5064 mL |
| 5 mM | 0.6101 mL | 3.0506 mL | 6.1013 mL |
| 10 mM | 0.3051 mL | 1.5253 mL | 3.0506 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)
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