>>Labeling & Detection>> Fluorescence>>Calcein Blue AM

Calcein Blue AM (Synonyms: Calcein Blue Acetoxymethyl ester)

Catalog No.GC18604

Calcein Blue AM은 세포염색제입니다.

Products are for research use only. Not for human use. We do not sell to patients.

Calcein Blue AM Chemical Structure

Cas No.: 168482-84-6

Size 가격 재고 수량
1mg
US$177.00
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Sample solution is provided at 25 µL, 10mM.

Description of Calcein Blue AM

Calcein Blue AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Blue AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Blue by intracellular esterases. Calcein Blue is retained by living cells and displays excitation/emission maxima of 360/445 nm, respectively. Calcein Blue AM is a blue-emitting variant of the green fluorophore Calcein AM , which has been used to evaluate cell viability and cytotoxicity.

Chemical Properties of Calcein Blue AM

Cas No. 168482-84-6 SDF
Synonyms Calcein Blue Acetoxymethyl ester
Chemical Name N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[(7-hydroxy-4-methyl-2-oxo-2H-1-benzopyran-8-yl)methyl]-glycine, (acetyloxy)methyl ester
Canonical SMILES OC1=CC=C(C(C)=CC(O2)=O)C2=C1CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
Formula C21H23NO11 M.Wt 465.4
Solubility DMSO : 6 mg/mL (18.68 mM) Storage Store at -20°C,protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of Calcein Blue AM

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.1487 mL 10.7434 mL 21.4869 mL
5 mM 0.4297 mL 2.1487 mL 4.2974 mL
10 mM 0.2149 mL 1.0743 mL 2.1487 mL
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**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

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In vivo Formulation Calculator (Clear solution) of Calcein Blue AM

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

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Average Rating: 5 ★★★★★ (Based on Reviews and 25 reference(s) in Google Scholar.)

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