Cas9 mRNA with N1-Me-pUTP (5'CAP) |
| Catalog No.GM10008 |
Cas9 mRNA with N1-Me-pUTP (5'CAP) is a DNA endonuclease mRNA produced by in vitro transcription. It has a Cap 1 cap structure and a poly(A) tail, and contains N1-Me-pUTP modification.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
Cas9 mRNA with N1-Me-pUTP (5'CAP) is produced through in vitro transcription. By simulating the mRNA processing process in eukaryotes, it has a 5' end Cap 1 cap structure and a 3' end poly(A) tail, which increases the stability and translation efficiency of the mRNA[1]. N1-methyl-pseudouridine (1-methylpseudouridine, m1ψ) is a methyl modification of naturally occurring pseudouridine, formed by N1-specific pseudouridine A that exists in archaea and eukaryotes It is catalyzed by the base transferase Nepl[2]. N1-methyl-pseudouridine is a substitute for uridine (U), which can effectively enhance RNA stability while reducing anti-RNA immune response [3].
Bacteria and archaea use the CRISPR/Cas system to defend against invading viruses and plasmids. The CRISPR/Cas system works in eukaryotic systems through two molecular components: Cas9 protein and non-coding guide RNA (gRNA). Cas9 endonuclease reaches the target site under the guidance of gRNA and cuts the sequence, resulting in gene silencing.
This product should be used in conjunction with purified Guide RNA.
References:
[1]. Jemielity J, Fowler T, Zuberek J, et al. Novel "anti-reverse" cap analogs with superior translational properties. RNA. 2003;9(9):1108-1122.
[2]. Callum J C Parr, et al.N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells. 2020 Apr 6;48(6):e35. doi: 10.1093/nar/gkaa070.
[3]. Pedro Morais, Hironori Adachi, Yi-Tao Yu.The Critical Contribution of Pseudouridine to mRNA COVID-19 Vaccines. 2021 Nov 4;9:789427. doi: 10.3389/fcell.2021.789427.
| mRNA Length | 4527 nucleotides | ||
| Concentration | 1mg/mL | ||
| Buffer | 1 mM Sodium Citrate, pH 6.4 | Storage | -40°C or below |
| General tips | Please dissolve it on ice and be careful to prevent RNase contamination and degradation. Avoid repeated freezing and thawing as much as possible. Do not vortex. For first use, gently centrifuge and divide into portions for individual use. Use RNase-free reagents and consumables, use appropriate RNase-free techniques, and do not add to the serum-containing medium until mixed with the transfection reagent. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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