DAF-2 (Synonyms: 4,5-Diaminofluorescein) |
Catalog No.GC43372 |
DAF-2는 세포와 조직의 산화질소(NO)에 대한 형광 검출기입니다.
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Cas No.: 205391-01-1
Sample solution is provided at 25 µL, 10mM.
DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO).[1],[2] It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively.2 At neutral pH the detection limit for NO is 2-5 nM.
Reference:
[1]. Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chemical & Pharmaceutical Bulletin 46, 373-375 (1998).
[2]. Nakatsubo, N., Kojima, H., Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Letters 427, 263-266 (1998).
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