Diethylmaleate |
| Catalog No.GC11776 |
Diethylmaleate는 말레산의 두 카르복시기와 에탄올의 형식 축합으로 인한 말레산 에스테르입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 141-05-9
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Diethylmaleate (DEM) is a maleic acid diethyl ester.
DEM is used in many organic syntheses as an additive and intermediate for pigments, plastics, pharmaceuticals, as well as agricultural products. DEM is also an intermediate for the production of adhesives, paints, and copolymers.
In vitro: Previous study found that the GSH depletion by DEM could inhibit the growth of transformed cells. The non-cytotoxic dose of DEM led to GSH depletion, cell cycle arrest, ROS generation, decrease in anchorage independent growth, apoptosis, gene expression changes as well as the activation of all three MAPK family members. In addition, the increased intracellular GSH levels by GSHe opposited the effect of DEM [1].
In vivo: Animal study was conducted to evaluate the effect of DEM induced oxidative stress on male fertility in mice. Results showed that the i.p. injection of DEM for two weeks led to decrease in reduced glutathione and increase in the oxidized glutathione levels in the testis. Moreover, the motility and concentration of sperm were found to be reduced significantly with a obvioius reduction in the litter size. It was also found that expression of redox sensitive transcription factor, cjun and cfos genes, gamma-glutamyl cysteine synthetase and manganese superoxide dismutase expression were affected after DEM treatment, as demonstrated by RT-PCR data. Additionally, the enzyme activity of SOD also increased by the treatment of DEM [2].
Clinical trial: Up to now, there is no clinical data reported for diethyl maleate.
References:
[1] Priya S,Nigam A,Bajpai P,Kumar S. Diethyl maleate inhibits MCA+TPA transformed cell growth via modulation of GSH, MAPK, and cancer pathways. Chem Biol Interact.2014 Aug 5;219:37-47.
[2] Kaur P,Kalia S,Bansal MP. Effect of diethyl maleate induced oxidative stress on male reproductive activity in mice: redox active enzymes and transcription factors expression. Mol Cell Biochem.2006 Oct;291(1-2):55-61.
| Cas No. | 141-05-9 | SDF | |
| Chemical Name | diethyl maleate | ||
| Canonical SMILES | CCOC(/C=C\C(OCC)=O)=O | ||
| Formula | C8H12O4 | M.Wt | 172.18 |
| Solubility | Ethanol : ≥ 100 mg/mL (580.79 mM); DMSO : 100 mg/mL (580.79 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 5.8079 mL | 29.0394 mL | 58.0788 mL |
| 5 mM | 1.1616 mL | 5.8079 mL | 11.6158 mL |
| 10 mM | 0.5808 mL | 2.9039 mL | 5.8079 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 36 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *