Dispase II

Dispase is neutral protease, also known as disperse enzyme, which is a non-specific metalloprotease. It is used to digest extracellular matrix from different tissues or organs, release and prepare primary single cells. Or used for cell harvesting or inoculation transfer in cell culture; Trypsin and Dispase, which are commonly used to prevent accidental cell agglomeration in suspension cell culture, are neutral proteases, also known as disperse enzymes, which are non-specific metalloproteinases used to digest extracellular matrix from different tissues or organs and release and prepare primary single cells. Or used for cell harvesting or inoculation transfer in cell culture; Trypsin, collagenase and pronase, which are also commonly used to prevent accidental cell agglomeration in suspension cell culture, belong to proteolytic enzymes, which are used to disperse tissues and separate cells, but they do great harm to cells, make them unstable during culture and even introduce mycoplasma pollution. Dispase will not damage the cell membrane, and because it comes from bacteria, it will not introduce mycoplasma or any animal virus; Differences in temperature, pH and serum components have little effect on Dispase. The activity of Dispase is greatly reduced after dilution, which is also convenient for subsequent culture. Collagenase and pronase belong to proteolytic enzymes, which are used to disperse tissues and separate cells, but they do great harm to cells, which are unstable in culture and even introduce mycoplasma pollution. Dispase will not damage the cell membrane, and because it comes from bacteria, it will not introduce mycoplasma or any animal virus; Differences in temperature, pH and serum components have little effect on Dispase. The activity of Dispase is greatly reduced after dilution, which is also convenient for subsequent culture. Advantages of Dispase: the enzyme acts quickly, effectively and gently; Cells can remain intact without damaging the cell membrane; The enzyme is of bacterial origin and does not contain mycoplasma or animal virus pollution; It is stable to temperature, pH change and serum composition. Simple inactivation can be achieved by using chelating agent or dilution.

The definition of enzyme activity: 1U is the amount of enzyme that releases an amount of amino acids or peptides equivalent to 1 umol of tyrosine per minute under the assay conditions; Dispase II: 50U/mg (at 37 degrees, using casein as the substrate, pH 7.5).

Inhibitors: EDTA, EGTA, Hg2+, and other heavy metals. Serum does not inhibit enzyme activity.

Usage: Dissolve Dispase in Hepes-buffered saline (50 mM Hepes/KOH pH 7.4, 150 mM NaCl) to a concentration of 10 mg/ml, and dilute with cell culture medium to 0.6-2.4 U/ml when in use. Working concentrations higher than 2.4 U/ml are not recommended. Sterilize by filtration through a 0.22 um sterile filter.