Emodin (Synonyms: Archin, Frangulic Acid, NSC 408120, NSC 622947, Schuttgelb) |
| Catalog No.GC16044 |
Natural CK2 inhibitor and ER agonist
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 518-82-1
Sample solution is provided at 25 µL, 10mM.
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| Cell experiment [1]: | |
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Cell lines |
Epithelial ovarian cancer (EOC) cells |
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Preparation method |
The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
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Reacting condition |
0 ~ 80 μM; 48 hrs |
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Applications |
In a series of EOC cells, Emodin inhibited cell proliferation in a dose-dependent manner. Emodin at the dose of 20 μM abrogated the migration and invasion abilities of A2780 and SK-OV-3 cells transfected with pLVX-ILK. The results of western blotting analysis implied that Emodin inhibited the migration and invasion abilities of EOC cells by blocking epithelial-mesenchymal transition (EMT) through targeting integrin-linked kinase (ILK). |
| Animal experiment [1]: | |
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Animal models |
Nude mice bearing SK-OV-3/pLVX-ILK cells |
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Dosage form |
50 mg/kg/d; i.p.; for 21 days |
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Applications |
In nude mice bearing SK-OV-3/pLVX-ILK cells, Emodin significantly inhibited tumor growth. Meanwhile, Emodin counteracted the effects of over-expression of ILK on EOC cells. The immunohistochemical results demonstrated that Emodin inhibited the EMT of tumor cells. In addition, Emodin exhibited mild cardiac, liver and renal toxicities in vivo. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Lu J, Xu Y, Zhao Z, Ke X, Wei X, Kang J, Zong X, Mao H, Liu P. Emodin suppresses proliferation, migration and invasion in ovarian cancer cells by down regulating ILK in vitro and in vivo. Onco Targets Ther. 2017 Jul 19;10:3579-3589. | |
| Cas No. | 518-82-1 | SDF | |
| Synonyms | Archin, Frangulic Acid, NSC 408120, NSC 622947, Schuttgelb | ||
| Chemical Name | 1,3,8-trihydroxy-6-methylanthracene-9,10-dione | ||
| Canonical SMILES | CC1=CC(O)=C2C(C(C3=CC(O)=CC(O)=C3C2=O)=O)=C1 | ||
| Formula | C15H10O5 | M.Wt | 270.24 |
| Solubility | ≥ 27 mg/mL in DMSO | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.7004 mL | 18.5021 mL | 37.0041 mL |
| 5 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL |
| 10 mM | 0.37 mL | 1.8502 mL | 3.7004 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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