Glyco-diosgenin |
| Catalog No.GC60879 |
Glyco-diosgenin은 구조 및 기능 연구와 막 단백질의 단일 입자 극저온 전자 현미경(cryoEM) 연구를 위해 막에서 단백질을 추출하기 위한 합성 계면활성제 및 세제입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1402423-29-3
Sample solution is provided at 25 µL, 10mM.
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Glyco-diosgenin is a synthetic surfactant and detergent for extracting proteins from membranes for structure and function studies, and single-particle cryo-electron microscopy (cryoEM) studies of membrane proteins[1][2].
Glyco-diosgenin (16 h) solubilizes and purifies the twin arginine translocation BC (TatBC) complex[1].
[1]. Dalsen L, et, al. In meso crystallogenesis. Compatibility of the lipid cubic phase with the synthetic digitonin analogue, glyco-diosgenin. J Appl Crystallogr. 2020 Mar 25;53(Pt 2):530-535. [2]. Wojnowska M,et, al. Precursor-Receptor Interactions in the Twin Arginine Protein Transport Pathway Probed with a New Receptor Complex Preparation. Biochemistry. 2018 Mar 13;57(10):1663-1671.
| Cas No. | 1402423-29-3 | SDF | |
| Canonical SMILES | C[C@@]([C@@]1([H])C2)(CC[C@@]3([H])[C@@]1([H])CC=C(C4)[C@@]3(CC[C@@H]4OCCC(CO[C@@H]([C@@H]([C@H]5O)O)O[C@@H]([C@H]5O[C@H]([C@@H]([C@H]6O)O)O[C@@H]([C@H]6O)CO)CO)CO[C@@H]([C@@H]([C@H]7O)O)O[C@@H]([C@H]7O[C@H]([C@@H]([C@H]8O)O)O[C@@H]([C@H]8O)CO)CO)C)[C@]9([H])[C@@]2([H])O[C@@](CC[C@H]%10C)(OC%10)[C@H]9C | ||
| Formula | C56H92O25 | M.Wt | 1165.31 |
| Solubility | DMSO: 100 mg/mL (85.81 mM) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.8581 mL | 4.2907 mL | 8.5814 mL |
| 5 mM | 0.1716 mL | 0.8581 mL | 1.7163 mL |
| 10 mM | 0.0858 mL | 0.4291 mL | 0.8581 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 12 reference(s) in Google Scholar.)
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