>>Signaling Pathways>> Ox Stress Reagents>> NO Donors>>JS-K

JS-K

Catalog No.GC18734

JS-K는 생리학적 pH에서 글루타티온과 반응하여 NO를 생성하는 NO 기증자입니다. JS-K는 Jurkat T 급성 림프모구성 백혈병 세포의 증식을 억제하고 세포자멸사를 유도하며 세포 주기를 방해합니다.

Products are for research use only. Not for human use. We do not sell to patients.

JS-K Chemical Structure

Cas No.: 205432-12-8

Size 가격 재고 수량
1mg
US$63.00
재고 있음
5mg
US$166.00
재고 있음

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Sample solution is provided at 25 µL, 10mM.

Description of JS-K

JS-K is a nitric oxide (NO) donor that reacts with glutathione to generate NO at physiological pH. It inhibits proliferation of HL-60 cells (IC50 = 0.5 uM), which is prevented by the glutathione precursor N-acetyl-L-cysteine , and inhibits growth of the solid tumor cell lines PPC-1, DLD-1, and Meth A. It decreases tumor volume by over 50% in an HL-60 mouse xenograft model when used at a dose of 4 uMol/kg, i.v., three times per week. JS-K inhibits proliferation, induces apoptosis, and disrupts the cell cycle of Jurkat T acute lymphoblastic leukemia cells. It also induces S-nitrosylation of β-catenin followed by dose-dependent degradation of nuclear β-catenin and S-nitrosylated nuclear β-catenin levels.

Chemical Properties of JS-K

Cas No. 205432-12-8 SDF
Chemical Name 4-[2-(2,4-dinitrophenoxy)-1-oxidodiazenyl]-1-piperazinecarboxylic acid, ethyl ester
Canonical SMILES O=[N](N1CCN(C(OCC)=O)CC1)=NOC(C=CC([N+]([O-])=O)=C2)=C2[N+]([O-])=O
Formula C13H16N6O8 M.Wt 384.3
Solubility DMF: 0.5 mg/ml,DMSO: 0.3 mg/ml,Ethanol: slightly soluble Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table of JS-K

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.6021 mL 13.0107 mL 26.0213 mL
5 mM 0.5204 mL 2.6021 mL 5.2043 mL
10 mM 0.2602 mL 1.3011 mL 2.6021 mL
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In vivo Formulation Calculator (Clear solution) of JS-K

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Product Documents

Quality Control & SDS

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Average Rating: 5 ★★★★★ (Based on Reviews and 8 reference(s) in Google Scholar.)

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