Lecithin (Synonyms: E 322, Lecithin (egg) , Lecithinon, Phospholutein, PtdCho, 3-fluoro UB) |
| Catalog No.GC30103 |
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Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 8002-43-5
Sample solution is provided at 25 µL, 10mM.
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Lecithin is regarded as a safe, conventional phospholipid source. Phospholipids are reported to alter the fatty acid composition and microstructure of the membranes in animal cells.
After culturing in MRS broth with 0.2 to 1.0% soy Lecithin, the survival rate of harvested cells increases significantly (P<0.05) in the 0.3% bile challenge compare with the no added soy Lecithin group. The cells incubated with 0.6% soy Lecithin are able to grow in an MRS broth with a higher bile salt content. The cell surface hydrophobicity is enhanced and the membrane integrity in the bile challenge increases after culturing with soy Lecithin. A shift in the fatty acid composition is also observed, illustrating the cell membrane changes in the soy Lecithin culture[1].
[1]. Hu B, et al. Enhancement of bile resistance in Lactobacillus plantarum strains by soy lecithin. Lett Appl Microbiol. 2015 Jul;61(1):13-9.
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Cell experiment: |
MRS broths are supplemented with soy Lecithin concentrations of 0, 0.2, 0.4, 0.6, 0.8 and 1.0%. Each broth is inoculated with a tested strain culture (2%, v/v) and anaerobically incubated at 37°C for 20 h. After incubation, the bacterium cells are harvested by centrifugation at 8000 g for 10 min at 4°C and washed twice in PBS (pH 6.5) plus ethanol (5%, v/v). Strain bile resistance is assessed. The numbers of viable cells are counted by the pouring plate method, and each batch is tested three times[1]. |
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References: [1]. Hu B, et al. Enhancement of bile resistance in Lactobacillus plantarum strains by soy lecithin. Lett Appl Microbiol. 2015 Jul;61(1):13-9. |
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| Cas No. | 8002-43-5 | SDF | |
| Synonyms | E 322, Lecithin (egg) , Lecithinon, Phospholutein, PtdCho, 3-fluoro UB | ||
| Canonical SMILES | O=P(OC[C@@H](COC(CCCCCCCCCCCCCCC)=O)OC(CCCCCCC/C=C\C/C=C\CCCCC)=O)(OCC[N+](C)(C)C)[O-] | ||
| Formula | C42H80NO8P | M.Wt | 758.06 |
| Solubility | DMSO : 5 mg/mL (6.60 mM);Water : 3.33 mg/mL (4.39 mM) | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3192 mL | 6.5958 mL | 13.1916 mL |
| 5 mM | 0.2638 mL | 1.3192 mL | 2.6383 mL |
| 10 mM | 0.1319 mL | 0.6596 mL | 1.3192 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 19 reference(s) in Google Scholar.)
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