MESG (7-methyl-6-Thioguanosine) (Synonyms: MESG) |
Catalog No.GC30262 |
MESG(7-methyl-6-Thioguanosine)(MESG)는 무기 인산염의 정량에 사용할 수 있는 발색 기질입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 55727-10-1
Sample solution is provided at 25 µL, 10mM.
7-methyl-6-Thioguanosine is a chromophoric substrate that is used to quantify inorganic phosphate (Pi). In the presence of purine nucleoside phosphorylase, it reacts with Pi to produce 7-methyl-6-thioguanine and ribose 1-phosphate, with the increase in 7-methyl-6-thioguanine followed by continuous spectrophotometry at an absorbance of 355-360 nm.1 7-methyl-6-Thioguanosine is also used to measure changes in Pi levels in coupled enzyme systems, including assays of the activities of protein phosphatases, phosphorylase kinases, GTPases, and ATPases.[1],[2],[3],[4]
Reference:
[1]. Webb, M.R. A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems. Proceedings of the National Academy of Sciences of the United States of America 89(11), 4884-4887 (1992).
[2]. Cheng, Q., Wang, Z.X., and Killilea, S.D. A continuous spectrophotometric assay for protein phosphatases. Analytical Biochemistry 310(2), 156-162 (2002).
[3]. Wang, Z.X., Cheng, Q., and Killilea, S.D. A continuous spectrophotometric assay for phosphorylase kinase. Analytical Biochemistry 230(1), 55-61 (1995).
[4]. Tan, Y.C., Wu, W.N., Zheng, Y., et al. Characterization of the interactions between the small GTPase RhoA and its guanine nucleotide exchange factors. Analytical Biochemistry 310(2), 156-162 (2014).
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