Name: Nigericin sodium salt
Brand: GlpBio
SKU: GC15811
Availability: InStock
Rating: 5 (26 reviews)

Product has been cited by 6 publications

Related Biological Data

Lysosomal accumulation and cellular pH-responsive capability of NRhD-PEG-4 NPs.(b) Fluorescence images of A549 and MCF-7 cells incubated with NRhD-PEG-4 NPs at different pH values (4.5, 5.5, 6.5, and 7.5).(c) Fluorescence quantification in (a).
Treatment of A549 and MCF-7 cells with high K+ buffer of 10.0 μM nigericin(GlpBio) for 30 min resulted in pHi calibration, and a red signal was detected that continually enhanced with the decreasing pHi.
Acta Biomaterialia (2022). PMID: 36087865 IF: 10.6335
Related Biological Data

Confocal fluorescence microscopic images of Hela cells stained with Hcy-Rh (10 μM in PBS, 60 min, 37 °C) and BODIPY (10 nM in PBS,10 min, 37 °C) upon further incubation with buffers containing high concentrations of K+ at different pH values.
Then, cells were further incubated with high-K+ buffers (30 mM NaCl, 120 mM KCl, 1 mM CaCl2, 0.5 mM MgSO4, 1 mM NaH2PO4, 5 mM glucose, 20 mM HEPES, and 20 mM NaOAc) of pH 6.0, 7.5,and 8.5 in the presence of 10.0 μM nigericin(GlpBio) for 20 min at 37 °C.
Analytical Chemistry (2022). PMID: 34989555 IF: 6.983
Related Biological Data

NLRP3 activation reversed the anti-pyroptosis effect of MRS2211 after gp120 treatment in PC12 cells. (B)After the combination of MRS2211 and nigericin, the NLRP3 expression was significantly higher than that in MRS2211- treated gp120 group, but significantly lower than that in nigericin treated group.
MRS2211(10 μmol/L) or/and nigericin (20 μmol/L, GLPBIO) were added to the medium for 30–45 min, respectively.
Neuropharmacology (2023): 109818. PMID: 38142931 IF: 4.7003
Related Biological Data

LNP/miR-22 suppresses hippocampal pyroptosis through NLRP3 in mice with I/R injury. The hippocampus pyroptosis was analyzed using double immunofluorescent stained with caspase-1 and TUNEL.
In the I/R+LNP/miR-22+Nigericin group, mice were administered both LNP/miR-22 through intraventricular stereotactic injection and NLRP3 activator Nigericin sodium salt(4 mg/kg, GLPBIO) intraperitoneally.
J Biomed Nanotechnol 20.3 (2024): 481-493. IF: 2.8997
Related Biological Data

Effect of Vitamin K on Pyroptosis in THP-1 Cells.(E–G) The LDH activity was measured in the supernatant. LPS plus nigericin (Nig) treatment was used to induce pyroptosis in THP-1 cells after 1 h pretreatment with Vitamin K1, K2 and K3.
Briefly, 1 × 10^6/mL cells were cultured with RPMI 1640 medium containing 2% FBS overnight and then treated with 1 µg/mL LPS for 3 h, followed by 8 µg/mL Nigericin(GlpBio) treatment for 1 h.
Biol Pharm Bull 46.1 (2023): 52-60. PMID: 36288961 IF: 2.2641

Product Documents

Quality Control & SDS

View current batch:

Purity: >98.00%

Protocol

세포 실험 [1]:
세포 라인	H460 세포
제조 방법	이동 및 침투 실험은 하룻밤 동안 세포에 세포 유동액을 주지 않은 상태에서 수행되었습니다. 이동 실험을 위해 H-460 세포는 Transwell 삽입물에 심어졌으며, RPMI 1640에서 약물입니나. Nigericin sodium salt (1μM)을 사용하거나 사용하지 않았습니다. 삽입물은 DMSO 또는 Nigericin sodium salt (1μM)이 포함된 10% FBS가 포함된 RPMI 1640의 접판 위에 놓였습니다. 24시간 후 세포 이동이 측정되었습니다.
반응 조건	1 µM Nigericin sodium salt ,24시간 동안
응용 분야	Nigericin sodium salt은 H460 폐암 세포의 이동과 침투를 억제하였습니다.
동물 실험 [2]:
동물 모형	누드 쥐（S18 세포가 누드 쥐의 어깨뼈 근처에 주사되었습니다）
제조 방법	쥐는 무작위로 네 그룹으로 나뉘어져 있으며 각 그룹은 쥐 6마리씩입니다. DDP(2.5 mg/kg)는 연속 5일간 복강내 주사로 투여되었고 Nigericin sodium salt (4 mg/kg)은 2일마다 복강내 주사로 투여되었습니다.
제형	매 2일마다 4 mg/kg의 Nigericin sodium salt.
응용 분야	Nigericin sodium salt은 종양 성장을 현저하게 감소시켰습니다.
참고문헌: [1]. Yakisich JS, Azad N, et,al. Nigericin decreases the viability of multidrug-resistant cancer cells and lung tumorspheres and potentiates the effects of cardiac glycosides. Tumour Biol. 2017 Mar;39(3):1010428317694310. doi: 10.1177/1010428317694310. PMID: 28351327. [2]. Deng CC, Liang Y, et,al. Nigericin selectively targets cancer stem cells in nasopharyngeal carcinoma. Int J Biochem Cell Biol. 2013 Sep;45(9):1997-2006. doi: 10.1016/j.biocel.2013.06.023. Epub 2013 Jul 4. PMID: 23831840.

Nigericin sodium salt은 Streptomyctus hygroscopicus에서 나오는 전기적으로 중성의 K+/H+ 이온 운송체로 지질을 선호하는 항생제로서 선별적으로 미토콘드 멤브레인에서 칼륨을 빼내는 기능을 가지고 있다. Nigericin sodium salt은 NLRP3 활성화제이다[1].

Nigericin sodium salt은 H460 폐암세포의 이동과 침투를 억제한다[1]. Nigericin sodium salt (0.1-10 nM)은 세포용량에 대한 명확한 이중효과를 가지고 있으며 낮은 농도에서는 수축효과를 높은 농도에서는 팽창효과를 나타낸다[2]. Nigericin sodium salt은 S18 세포에 대한 독성이 S26 세포보다 높으며 각각의 IC50 값은 2.03±0.55 μM와 4.77±2.35 μM이다. Nigericin sodium salt은 S18 및 HONE-1 세포의 이동 능력을 현저하게 낮추었다[3]. Nigericin sodium salt은 HT29 및 SW116 세포계에 대한 독성을 나타내며 각각의 IC50 값은 12.92±0.25 μmol과 15.86±0.18 μmol이다. Nigericin sodium salt은 또한 표준 소프트 에이거 시험에서 댐핑되지 않은 조건에서 콜로니 형성을 하는 능력이 감소했다[4]. 활성 세포 영상 및 생화학적 접근법을 결합하여 전형적인 NLRP3 자극물인 칼륨 이온 운송체 니게릭인이나 밀리몰 농도의 ATP는 거대세포에 NLRP3 독립적 및 의존적인 효과를 미치며 사이토크인 릴리스 및 세포 사망으로 이어진다[5]. MN9D 세포에 Nigericin sodium salt을 처리하면 LC3-II 및 p62 수준이 증가하고 동시에 캐스파이스가 활성화되었다. 초미세구조 검사에서 니게릭인 처리된 세포에서 자포성 포도와 팽창된 포도의 축적을 발견했다[7].

쥐에서 니게릭인은 종양 성장을 현저하게 감소시킨다. Nigericin sodium salt은 체내에서 Bmi-1의 표현을 현저하게 낮추었다. Bmi-1의 과표현은 Nigericin sodium salt 처리로 인한 NPC 세포의 CSC 함량과 전이 능력을 부분적으로 회복시킨다. Bmi-1의 다운조절은 Nigericin sodium salt이 NPC의 CSC에 대한 억제 효과에 관련될 수 있다[3]. Nigericin sodium salt은 STAT3을 직접적으로 타겟팅하여 인간 뼈 악성종양 세포에 항암 효과를 발휘한다. Nigericin sodium salt은 종양의 이동과 침투를 현저하게 억제할 수 있다. Nigericin sodium salt은 쥐 뼈 악성종양 모델에서 종양 성장을 억제한다[6].

References:
[1]: Yakisich JS, Azad N, et,al. Nigericin decreases the viability of multidrug-resistant cancer cells and lung tumorspheres and potentiates the effects of cardiac glycosides. Tumour Biol. 2017 Mar;39(3):1010428317694310. doi: 10.1177/1010428317694310. PMID: 28351327.
[2]: Bissinger R, Malik A, et,al. Triggering of Suicidal Erythrocyte Death by the Antibiotic Ionophore Nigericin. Basic Clin Pharmacol Toxicol. 2016 May;118(5):381-9. doi: 10.1111/bcpt.12503. Epub 2015 Nov 13. PMID: 26458067.
[3]: Deng CC, Liang Y, et,al. Nigericin selectively targets cancer stem cells in nasopharyngeal carcinoma. Int J Biochem Cell Biol. 2013 Sep;45(9):1997-2006. doi: 10.1016/j.biocel.2013.06.023. Epub 2013 Jul 4. PMID: 23831840.
[4]: Zhou HM, Dong TT, et,al. Suppression of colorectal cancer metastasis by nigericin through inhibition of epithelial-mesenchymal transition. World J Gastroenterol. 2012 Jun 7;18(21):2640-8. doi: 10.3748/wjg.v18.i21.2640. PMID: 22690072; PMCID: PMC3370000.
[5]: Heid ME, Keyel PA, et,al. Mitochondrial reactive oxygen species induces NLRP3-dependent lysosomal damage and inflammasome activation. J Immunol. 2013 Nov 15;191(10):5230-8. doi: 10.4049/jimmunol.1301490. Epub 2013 Oct 2. PMID: 24089192; PMCID: PMC3833073.
[6]: Yang Z, Xie J, et,al. Nigericin exerts anticancer effects through inhibition of the SRC/STAT3/BCL-2 in osteosarcoma. Biochem Pharmacol. 2022 Apr;198:114938. doi: 10.1016/j.bcp.2022.114938. Epub 2022 Jan 31. PMID: 35114189.
[7]: Lim J, Lee Y, et,al. Nigericin-induced impairment of autophagic flux in neuronal cells is inhibited by overexpression of Bak. J Biol Chem. 2012 Jul 6;287(28):23271-82. doi: 10.1074/jbc.M112.364281. Epub 2012 Apr 5. PMID: 22493436; PMCID: PMC3390606.
[8]: Fruth IA, Arrizabalaga G. Toxoplasma gondii: induction of egress by the potassium ionophore nigericin. Int J Parasitol. 2007 Dec;37(14):1559-67. doi: 10.1016/j.ijpara.2007.05.010. Epub 2007 Jun 9. PMID: 17618633; PMCID: PMC2221775.
[9]:Kucejova B, Kucej M, et,al. A screen for nigericin-resistant yeast mutants revealed genes controlling mitochondrial volume and mitochondrial cation homeostasis. Genetics. 2005 Oct;171(2):517-26. doi: 10.1534/genetics.105.046540. Epub 2005 Jul 14. PMID: 16020778; PMCID: PMC1456768.

Cas No.	28643-80-3	SDF
Synonyms	NSC 292567
Formula	C₄₀H₆₇NaO₁₁	M.Wt	746.94
Solubility	25mg/mL in EtOH	Storage	Store at -20°C
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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		1 mg	5 mg	10 mg
	1 mM	1.3388 mL	6.694 mL	13.388 mL
	5 mM	0.2678 mL	1.3388 mL	2.6776 mL
	10 mM	0.1339 mL	0.6694 mL	1.3388 mL

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Nigericin sodium salt (Synonyms: NSC 292567)

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Product has been cited by 6 publications

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Quality Control & SDS

Protocol

Complete Stock Solution Preparation Table

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Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

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