Ophiobolin B (Synonyms: Cochliobolin B,Ophiobolsin A,Zizanin B) |
| Catalog No.GC15233 |
Helminthosporium oryzae의 sesterterpene 대사 산물인 Ophiobolin B는 옥수수 딱정벌레로부터 양성자 압출을 억제합니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 5601-74-1
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Ophiobolins are a group of sesterterpene-type phytotoxins produced by fungi belonging to the genera Bipolaris, Drechslera, Cephalosporium and Aspergillus. Ophiobolins are secondary metabolites of fungi. To date, more than 25 ophiobolin analogues have been identified. Ophiobolins have been involved in various biological actions, such as phytotoxic, cytotoxic, nematocidal, antimicrobial and antiviral effects [1].
In vitro: In maize coleoptile tissues, Ophiobolin B inhibited proton extrusion. Ophiobolin B counteracted the biological activity of fusicoccin (FC). Ophiobolin B inhibited FC-promoted proton extrusion, potassium uptake and cell enlargement [2]. Calmodulin solutions preincubation with ophiobolin A caused an instantaneous quenching of the intrinsic tyrosine fluorescence in a time-dependent manner. The inhibitory effects of ophiobolin A could not be reversed by dialysis, dilution, nor denaturation by urea in the presence of methanol followed by renaturation. Ophiobolin A also inhibited spinach calmodulin [3]. In cultured CLL cells, treatment with increasing concentrations of Ophiobolin B for 24 h displayed bioactivity towards leukemia cells with induction of apoptosis at nanomolar concentrations [3]. Ophiobolins B exihibited antifungal effects on different zygomycetes with MIC value of 25-50 μg/ml [4].
References:
[1] Au T K, Chick W S H, Leung P C. The biology of ophiobolins[J]. Life sciences, 2000, 67(7): 733-742.
[2] Gianani L, Cocucci S, Pardi D, et al. Effects of ophiobolin B on cell enlargement and H+/K+ exchange in maize coleoptile tissues[J]. Planta, 1979, 146(3): 271-274.
[3] Bladt T T, Dürr C, Knudsen P B, et al. Bio-activity and dereplication-based discovery of ophiobolins and other fungal secondary metabolites targeting leukemia cells[J]. Molecules, 2013, 18(12): 14629-14650.
[4] Krizsán K, Bencsik O, Nyilasi I, et al. Effect of the sesterterpene-type metabolites, ophiobolins A and B, on zygomycetes fungi[J]. FEMS microbiology letters, 2010, 313(2): 135-140.
| Cas No. | 5601-74-1 | SDF | |
| Synonyms | Cochliobolin B,Ophiobolsin A,Zizanin B | ||
| Chemical Name | (1R,3aS,6aR,7S,9aR,10aS)-7-[(1S)-1,5-dimethyl-4-hexen-1-yl]-1,2,3,3a,6,6a,7,8,9,9a,10,10a-dodecahydro-1,7-dihydroxy-1,9a-dimethyl-3-oxo-dicyclopenta[a,d]cyclooctene-4-carboxaldehyde | ||
| Canonical SMILES | C[C@@]12C[C@@]([H])([C@]3(/C(C=O)=C\C[C@]1([C@](O)(CC2)[C@H](CC/C=C(C)\C)C)[H])[H])[C@](CC3=O)(C)O | ||
| Formula | C25H38O4 | M.Wt | 402.6 |
| Solubility | DMF: soluble,DMSO: soluble,Ethanol: soluble,Methanol: soluble | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4839 mL | 12.4193 mL | 24.8385 mL |
| 5 mM | 0.4968 mL | 2.4839 mL | 4.9677 mL |
| 10 mM | 0.2484 mL | 1.2419 mL | 2.4839 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 16 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *