O-Propargyl-Puromycin (O-Propargylpuromycin) (Synonyms: OP-puro) |
Catalog No.GC30010 |
퓨로마이신의 알킨 유사체인 O-프로파길-퓨로마이신(O-Propargylpuromycin)은 강력한 단백질 합성 억제제입니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1416561-90-4
Sample solution is provided at 25 µL, 10mM.
O-propargyl-puromycin, an alkyne analog of puromycin, is a potent protein synthesis inhibitor.
O-propargyl-puromycin can be used to image and affinity-purify nascent proteins in cells and in animals. O-propargyl-puromycin inhibits protein synthesis, both in reticulocyte lysates and in cultured cells, displaying a potency two- to threefold lower than that of unmodified puromycin. O-propargyl-puromycin forms covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition. [1].
Tissues from uninjected mice shows low nonspecific staining, whereas tissues from O-propargyl-puromycin-injected mice display specific patterns of O-propargyl-puromycin incorporation into nascent proteins. In the small intestine, translation is strongest in cells in the crypts and at the base of intestinal villi, consistent with the high proliferative and secretory activity of these cells. The stain is particularly strong in Paneth cells, which are located close to the base of the crypts and are filled with secretory vesicles. The intense O-propargyl-puromycin labeling of vesicles in Paneth cells suggests that prematurely terminated, O-propargyl-puromycin-conjugated secretory proteins are translocated into the endoplasmic reticulum (ER) lumen[1].
[1]. Liu J, et al. Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):413-8.
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