Thiazinamium chloride (Multergan chloride) |
| Catalog No.GC31807 |
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 4320-13-2
Sample solution is provided at 25 µL, 10mM.
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Thiazinamium chloride possesses potent anticholinergic and antiallergic activity and inhibits TxB2 synthesis with IC50 value of 0.2 µM.
TxB2 synthesis by resting macrophages is inhibited by thiazinamium chloride and promethazine in a dose-dependent manner. Thiazinamium chloride inhibits TxBz synthesis but had no effect on the ingestion of zymosan particles. In contrast, chlorpromazine inhibits phagocytosis but not TxBz synthesis except at lo-3 M. Under the condition where indomethacin, a nown cyclooxygenase inhibitor, is inhibitory, promethazine but not thiazinamium chloride inhibits TxB2 synthesis from exogenous arachidonic acid. Treatment of macrophages with promethazine and chlorpromazine but not thiazinamium chloride results in a reduction in the oxidative burst during phagocytosis. Furthermore, the ability of thiazinamium chloride to selectively inhibit arachidonic acid metabolism may contribute to its bronchodilator/antiallergic activity[1].
[1]. Chang J, et al. Effects of thiazinamium chloride, promethazine and chlorpromazine on thromboxane B2 synthesis, phagocytosis and respiratory burst by rat alveolar macrophages. Biochem Pharmacol. 1983 Sep 15;32(18):2671-7.
Cell experiment: | Rats are anesthetized with an intraperitoneal injections of sodium pentobarbital (43mg/kg body wt) and exsanguinated by cardiac puncture; their lungs are isolated and lavaged with a total of 50mL of lavage solution. Lungs that exhibit infection or gross pathological changes are not used. The lavage fluid containing macrophages is centrifuged at 300g for 10 min, and the cell pellet is resuspended in serum free M199. Macrophage monolayers are established by incubating 1.5x106 cells in petri dishes (35x10mm) for 2 hr in an atmosphere of 95% room air and 5% carbon dioxide. After washing with I-IBSS to remove non-adherent cells, the cultures are incubated in serum-free Ml99 with or without zymosan (100μg/mL) in the absence or presence of drugs(including Thiazinamium chloride)[1]. |
References: [1]. Chang J, et al. Effects of thiazinamium chloride, promethazine and chlorpromazine on thromboxane B2 synthesis, phagocytosis and respiratory burst by rat alveolar macrophages. Biochem Pharmacol. 1983 Sep 15;32(18):2671-7. | |
| Cas No. | 4320-13-2 | SDF | |
| Canonical SMILES | C[N+](C)(C)C(C)CN1C2=C(C=CC=C2)SC3=CC=CC=C13.[Cl-] | ||
| Formula | C18H23ClN2S | M.Wt | 334.91 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.9859 mL | 14.9294 mL | 29.8588 mL |
| 5 mM | 0.5972 mL | 2.9859 mL | 5.9718 mL |
| 10 mM | 0.2986 mL | 1.4929 mL | 2.9859 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)
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