>>Kits>>TRIzol NC Reagent

TRIzol NC Reagent

Catalog No.GK20010

TRIzol NC Reagent is a total RNA extraction reagent that without using chloroform, and is widely suitable for cultured cells, animal tissues, and plant tissues.

Products are for research use only. Not for human use. We do not sell to patients.

TRIzol NC Reagent Chemical Structure

Size 가격 재고 수량
100 rxns
US$100.00
재고 있음
200 rxns
US$180.00
재고 있음

Tel:(909) 407-4943 Email: sales@glpbio.com


고객 리뷰

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Description Protocol Components Features & Properties Product Documents

TRIzol NC Reagent is a total RNA extraction reagent that without using chloroform, and is widely suitable for cultured cells, animal tissues, and plant tissues.

The extraction process takes about 1 hour, and the total RNA obtained can be directly used in molecular biology experiments such as RT-PCR, qRT-PCR, Northern Blot, Dot Blot, in vitro translation, and high-throughput sequencing.

It is recommended to use the SuperFast RT Master Mix for qPCR (gDNA remover) (Cat.No. GK10031) reverse transcription kit and SYBR Green qPCR Master Mix (Cat.No. GK10002) real-time fluorescence quantitative PCR kit for subsequent experiment.

Sample size and RNA yield

Sample type

Sample size

RNA yield

Leukocyte

1x106 cells

10~20μg

Plant tissue

50mg

10~25μg

Cells

1x106 cells

10-30μg

Tissues such as muscle/brain

50mg

10-25μg

Liver

50mg

100~300μg

 

This product is a blue solution. During the RNA extraction process, impurities such as proteins, polysaccharides, and DNA can be precipitated to the bottom of the tube with an obvious blue precipitate layer, which is convenient for absorbing RNA supernatant, effectively removing impurities, therefore ensuring the integrity and purity of RNA. Compared with traditional TRIzol methods, The same 50mg tissue sample needs to add 0.5ml TRIzol NC Reagent, while the traditional TRIzol Reagent needs to add 1ml, so as it saves reagent. TRIzol NC Reagent is more easy to operate, can be operated at room temperature throughout the process, and does not require chloroform for phase separation,  with higher security.

TRIzol NC Reagent

Fig1. Comparison of TRIzol NC operation process with conventional Trizol operation process

 

FAQ:

1.Q: The traditional TRIzol method requires centrifugation at 4℃ for RNA extraction, but does this product extract RNA at room temperature?

A: Yes, TRIzol NC Reagent can extract RNA at room temperature, and the composition of the lysate can fully protect RNA from degradation. It is better if operated on ice and at 4 ℃.

 

2.Q: Is there stratification after the TRIzol NC is added to the diluent and centrifuged?

A: After step 2.2 centrifugation, obvious blue bottom precipitate and RNA supernatant can be seen.

 

3.Q: There was no pellet after adding isopropyl alcohol?

A: Usually, a white gelatinous pellet can be seen after isopropyl alcohol precipitation. If the pellet is not visible, it may be due to low RNA content or low tissue dosage. It is recommended to increase the amount of sample and TRIzol NC Reagent. When the supernatant is abandoned in steps 2.5-2.8, the method of absorbing rather than dumping is adopted to avoid the loss of pellet; The upper solution was taken and 5-10μg RNase-free glycogen was added as carrier for co-precipitation with RNA. In addition, some tissue materials contain more metabolites, resulting in precipitation could not accumulate and dispersed on the centrifugal tube wall, at this time, please slowly absorb supernatant followed by the liquid surface, after 75% ethanol washing RNA precipitation, white pellet will become obvious.

 

4.Q: Which samples are suitable for the product?

A: TRIzol NC Reagent can extract cell samples, animal and plant tissue samples. Not applicable to blood samples, not applicable to animal white fat tissue. TRIzol LS Reagent (Cat.No. GK20009) is recommended for blood samples. TRIzol Reagent (Cat.No. GK20008) is recommended for animal white fat tissue samples.

리뷰

Review for TRIzol NC Reagent

Average Rating: 5 ★★★★★ (Based on Reviews and 30 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for TRIzol NC Reagent

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.