XAV-939

Cell lines

A549 cells

Preparation Method

A549 cells in the logarithmic growth phase were seeded in 96-well plates, at a density of 2×10⁴ cells/well, and subsequently treated with various XAV-939 concentrations (0.1, 0.5, 1, 5, 10 µmol/l) for 24, 48, 72 and 96 h.

Reaction Conditions

0.1, 0.5, 1, 5, 10 µmol/l for 24, 48, 72 and 96 h

Applications

At all experimental time points, XAV-939 treatment was able to significantly inhibit A549 cell proliferation compared with the control group (F24h=30.382, F48h=52.463, F72h=56.635, F96h=59.274), with the exception of the 5-and 10-µmol/l groups at the 24 h time point.

Animal models

DBA/2 inbred mice

Preparation Method

Mice received an intraperitoneal injection of 100 µl of XAV-939 (2 mg/ml) along with a 10% dimethyl sulfoxide (DMSO; compounded by 0.9% NaCl solution) every day since they received vaccination with the L1210 cells, while according to other experimental groups, mice received intraperitoneal injections along with 100 µl of 10% DMSO solution (compounded by 0.9% NaCl solution) for 21 days continuously.

Dosage form

Intraperitoneal injection, 100 µl of 2 mg/ml for 21 days

Applications

In the model, NC, HOTAIR mimics, siRNA HOTAIR, XAV-939, and HOTAIR mimics + XAV-939 groups, the WBC number in PB increased on the 21st day by 341.08, 355.72, 499.85, 196.49, 187.48, and 363.35%. However, the PLT number decreased by 66.37, 69.46, 81.27, 49.24, 50.47, and 72.61%; hemoglobin content decreased by 39.02, 41.05, 60.52, 14.01, 17.20, and 42.36%.

References:

[1]: Li C, Zheng X, Han Y, Lv Y, Lan F, Zhao J. XAV939 inhibits the proliferation and migration of lung adenocarcinoma A549 cells through the WNT pathway. Oncol Lett. (2018) 15:8973-82. doi: 10.3892/ol.2018.8491
[2]: Li GJ, Ding H and Miao D: Long-noncoding RNA HOTAIR inhibits immunologic rejection of mouse leukemia cells through activating the Wnt/β-catenin signaling pathway in a mouse model of leukemia. J Cell Physiol. 234:10386-10396. 2019.