Levomefolate-13C-d3 (Synonyms: L-Methyl Folate-13C-d3, 6S-5-Methyltetrahydrofolate-13C-d3, 6S-5-MTHF-13C-d3) |
| Catalog No.GC49020 |
An internal standard for the quantification of levomefolate
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1356019-94-7
Sample solution is provided at 25 µL, 10mM.
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Levomefolate-13C-d3 is intended for use as an internal standard for the quantification of levomefolate by GC- or LC-MS. Levomefolate is the single (S) isomer of 5-methyltetrahydrofolic acid and an endogenous active form of folic acid .1 It donates a methyl group to homocysteine in the biosynthesis of methionine.2 Levomefolate (2 mg/kg) prevents decreases in circulating polymorphonucleates induced by methotrexate in an L1210 murine skin lymphocytic leukemia model, and it increases survival in the same model when administered in combination with methotrexate.3 Formulations containing levomefolate have been used as dietary supplements, as well as in oral contraceptives for the prevention of neural tube defects in unintended pregnancies.
1.Wang, X., Shen, F., Freisheim, J.H., et al.Differential stereospecificities and affinities of folate receptor isoforms for folate compounds and antifolatesBiochem. Pharmacol.44(9)1898-1901(1992) 2.Ragsdale, S.W.Catalysis of methyl group transfers involving tetrahydrofolate and B12Vitam. Horm.79293-324(2008) 3.Simile, M.M., DeMiglio, M.R., Nufris, A., et al.l-5-formyltetrahydrofolate and l-5-methyltetrahydrofolate rescue in L1210 leukemia treated with high methotrexate dosesRes. Commun. Chem. Pathol. Pharmacol.81(2)251-254(1993)
| Cas No. | 1356019-94-7 | SDF | |
| Synonyms | L-Methyl Folate-13C-d3, 6S-5-Methyltetrahydrofolate-13C-d3, 6S-5-MTHF-13C-d3 | ||
| Canonical SMILES | O=C1C2=C(NC[C@H](CNC3=CC=C(C(N[C@H](C(O)=O)CCC(O)=O)=O)C=C3)N2[13C]([2H])([2H])[2H])NC(N)=N1 | ||
| Formula | C19[13C]H22D3N7O6 | M.Wt | 463.5 |
| Solubility | H2O : 2.08 mg/mL (4.49 mM; ultrasonic and warming and heat to 60°C) | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1575 mL | 10.7875 mL | 21.575 mL |
| 5 mM | 0.4315 mL | 2.1575 mL | 4.315 mL |
| 10 mM | 0.2157 mL | 1.0787 mL | 2.1575 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)
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