LiCl |
| Catalog No.GC12077 |
used to precipitate RNA
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 7447-41-8
Sample solution is provided at 25 µL, 10mM.
IC50: N/A
LiCl is a typical ionic compound, though the small size of the Li+ ion makes its properties not seen for other alkali metal chlorides, such as the extraordinary solubility in polar solvents and hygroscopic properties.
In vitro: Though precipitation with a monovalent cation or alcohol is much more widely used, LiCl has been frequently used to precipitate RNA. LiCl precipitation gives various advantages over other methods: LiCl does not precipitate DNA, protein and carbohydrate efficiently. LiCl can be also used for removing translation inhibitors or cDNA synthesis from RNA preparations. LiCl can be also used as a simple rapid method for recovering RNA from in-vitro transcription reactions [1]. In addition, a method for isolation of large, translationally active RNA samples has been presented. In this study, the procedure included homogenization of samples in 5 m guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 m LiCl. The advantages of this method include simplicity, speed, avoidance of ultracentrifugation, and its applicability to large numbers of samples. The method yielded large mRNA precursors up to 10 kb [2].
In vivo: Currently, there is no animal data reported.
Clinical trial: Up to now, LiCl has not been used clinically.
References:
[1] BARLOW JJ, MATHIAS AP, WILLIAMSON R, GAMMACK DB. A simple method for the quantitative isolation of undegraded high molecular weight ribonucleic acid. Biochem Biophys Res Commun.1963 Sep 10;13:61-6.
[2] Cathala, G. , Savouret, J., Mendez, B., West, B. L., Karin, M., Martial, J. A., and Baxter, J. D. (1983). "A Method for Isolation of Intact, Translationally Active Ribonucleic Acid".DNA.2(4): 329–335.
| Cas No. | 7447-41-8 | SDF | |
| Chemical Name | lithium chloride | ||
| Canonical SMILES | [Cl-].[Li+] | ||
| Formula | ClLi | M.Wt | 42.39 |
| Solubility | ≥ 4.2mg/mL in Water | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 23.5905 mL | 117.9523 mL | 235.9047 mL |
| 5 mM | 4.7181 mL | 23.5905 mL | 47.1809 mL |
| 10 mM | 2.359 mL | 11.7952 mL | 23.5905 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
-
Related Biological Data
The effect of E5 on apoptosis and cell migration in colorectal cancer cells.(G-H) Transwell migration assays were carried out.
DLD-1 cells were exposed to different treatments for 24 h, including E5 (2 μM) and E5 (2 μM) +LiCl (20 mmol/L)(GlpBio).
Process Biochemistry, 2023. IF: 4.3997 -
Related Biological Data
The C1s enzyme suppresses foam cell formation by cleaving LRP5/6.
To evaluate the LRP5 level in cell lysate and culture medium, RAW 264.7 cells were treated with LiCl(GlpBio), C1s enzyme or ox-LDL for 24 h.
Mol Immunol 166 (2024): 29-38. PMID: 38218080 IF: 3.5999
Average Rating: 5 (Based on Reviews and 23 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *