Home >> Limited Time Event

Buy GK10002/GK10031 and Get a Free GK10031/GK10002

The purchase-and-get-one-free promotion ends on July 31, 2024.


Note: Will provide                 free products if order through https://www.fishersci.com


☆ GK10002 : SYBR Green qPCR Master Mix
☆ GK10031 : SuperFast RT Master Mix for qPCR (gDNA remover)

Buy either one to get another for free
GK10002 -5mL (500 rxns) $70.00 GK10031-50 rxns $65.00
GK10002 -10mL (1000 rxns) $110.00 GK10031-100 rxns $102.00
GK10002 -25mL (2500 rxns) $250.00 GK10031-250 rxns $245.00
Reverse Transcription Kit Introduction

SuperFast RT Master Mix for qPCR (gDNA remover) is a highly sensitive reverse transcription kit. The cDNA synthesized by this kit is the first step of the two-step RT-PCR experiment. The obtained cDNA can be used for conventional PCR or RT-PCR.

SuperFast RT Master Mix for qPCR (gDNA remover) uses a high-temperature resistant (>50°C) ultra-fast amplification reverse transcriptase which can synthesize cDNA from extremely low amounts (pg~μg level) of total RNA or poly(A) mRNA and is compatible with RNA templates with high GC content and complex secondary structure.

In many cases RNA extracted by methods such as spin columns or acid guanidine-phenol chloroform (AGPC) will contain a small amount of genomic DNA (gDNA). When pseudogenes exist in the target gene or primers cannot be designed across introns genomic DNA will be amplified together with cDNA thus affecting the accuracy of the data. SuperFast RT Master Mix for qPCR (gDNA remover) contains a gDNA remover with strong DNA degradation ability which helps to remove gDNA and reverse transcribe RNA without purification.

Features
Efficient and fast

gDNA removal and cDNA synthesis can be completed in just 8-10 minutes.

Ultra-high sensitivity

Good reverse transcription reaction can be performed even with very small amounts of RNA template.

Super strong compatibility

Compatible with RNA templates with high GC content and complex secondary structure.

Ultra-low impact factors

Super strong applicability to subsequent qPCR reactions: Through continuous optimization, the impact of this kit on subsequent qPCR reactions is minimized.

The figure below shows the process and difference between GLPbio and conventional reverse transcription kits. Thanks to this highly efficient reverse transcriptase, Glpbio's reverse transcription kit completes the reverse transcription process only in 8 minutes, which greatly shortens the time compared to conventional reverse transcription.

SuperFast RT Master Mix for qPCR (gDNA remover) is a reverse transcription kit designed for researchers who pursue extreme efficiency and precision. It not only provides unparalleled reverse transcription effects, but also ensures the purity and accuracy of experimental results through a unique gDNA removal mix.

To further optimize the experimental process, SuperFast RT Master Mix can be used in conjunction with our other star product, SYBR Green qPCR Master Mix. This combination not only improves the sensitivity of the experiment, but also makes each peak of the data clearly visible through its excellent fluorescence enhancement effect.

Please note that the purchase-and-get-one-free promotion ends on July 31, 2024. Hurry up and experience the efficient reverse transcription and smooth fluorescent quantitative qPCR.