N-Acetylserotonin (Synonyms: N-Acetyl-5-hydroxytryptamine,NAS) |
| Catalog No.GC15365 |
N-Acetylserotonin is a Melatonin precursor, and that it can potently activate TrkB receptor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1210-83-9
Sample solution is provided at 25 µL, 10mM.
N-Acetylserotonin is an agonist at the melatonin receptors MT1, MT2, and MT3.
A melatonin receptor is a G protein-coupled receptor binding melatonin. Three types of melatonin receptor have been identified. The MT1 and MT2 receptor subtypes are in humans and other mammals, whereas an additional melatonin receptor subtype MT3 has been identified in amphibia and birds.
In vitro: N-Acetylserotonin, a precursor of melatonin, was acetylated from serotonin by arylalkylamine Nacetyltransferase (AANAT). N-Acetylserotonin was found to be able to swiftly activate TrkB in a circadian manner. N-Acetylserotonin also exhibited antidepressant effect in a TrkB-dependent manner. In additioin, N-acetylserotonin could rapidly activate TrkB, but not TrkA or TrkC, in a neurotrophin- and MT3 receptor-independent manner. Moreover, N-acetylserotonin, but not melatonin, showed a robust antidepressant-like behavioral effect in a TrkB-dependent way [1].
In vivo: Animal study found that in BDNF knockout mice the administration of N-acetylserotonin could activate TrkB. Moreover, the endogenous TrkB was activated in wild-type C3H/f+/+ mice but not in AANAT-mutated C57BL/6J mice, in a circadian rhythm. In addition, TrkB activation was found to be high at night in the dark and low during the day [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Jang, S. W.,Liu, X.,Pradoldej, S., et al. N-acetylserotonin activates TrkB receptor in a circadian rhythm. Proceedings of the National Academy of Sciences of the United States of America 107(8), 3876-3881 (2010).
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Animal experiment: |
Mice[1] Two-month-old TrkB F616A mice are pretreated with 1NMPP1 in drinking water (50 μM) 1 day before the experiment, followed by administration of N-Acetyl-5-hydroxytryptamine (20 mg/kg, i.p.) or Melatonin (1 mg/kg, i.p.). Mice are killed at 1 h. The brain homogenates are analyzed by immunoblotting with anti-p-TrkB. Two- to three-month-old BDNF forebrain conditional knockout mice are injected i.p. with N-Acetyl-5-hydroxytryptamine or Melatonin. Mice are killed at 0, 0.5, 1, or 2 h following drug administration. Brain lysates are prepared and analyzed by immunoblotting with anti-phospho-TrkB Y816[1]. |
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References: [1]. Jang SW, et al. N-acetylserotonin activates TrkB receptor in a circadian rhythm. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3876-81. |
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| Cas No. | 1210-83-9 | SDF | |
| Synonyms | N-Acetyl-5-hydroxytryptamine,NAS | ||
| Chemical Name | N-[2-(5-hydroxy-1H-indol-3-yl)ethyl]-acetamide | ||
| Canonical SMILES | OC1=CC=C(NC=C2CCNC(C)=O)C2=C1 | ||
| Formula | C12H14N2O2 | M.Wt | 218.3 |
| Solubility | ≥ 11.5mg/mL in ethanol | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.5809 mL | 22.9043 mL | 45.8085 mL |
| 5 mM | 0.9162 mL | 4.5809 mL | 9.1617 mL |
| 10 mM | 0.4581 mL | 2.2904 mL | 4.5809 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 34 reference(s) in Google Scholar.)
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